Visualization of dystrophic muscle fibers in Mdx mouse by vital staining with Evans blue: Evidence of apoptosis in dystrophin-deficient muscle

Ryoichi Matsuda, Akio Nishikawa, Hideho Tanaka

Research output: Contribution to journalArticle

256 Citations (Scopus)

Abstract

Degenerating muscle fibers in the skeletal muscle of mdx mice were visualized by vital staining with Evans blue. Evans blue injected intravenously stained only degenerating muscle fibers which were visible as blue fibers macroscopically and could also be seen as red fluorescent fibers microscopically. Evans blue-stained muscle fibers were either hypercontracted or degrading. Intact or regenerating muscle fibers in mdx mice and muscle fibers in B10 control mice were not stained with the dye. DNA isolated from Evans blue-stained fibers exhibited fragmentation to approximately 180 base pairs on agarose gel electrophoresis. Such DNA fragmentation was not found in DNA from unstained muscle fibers in mdx or B10 mice. Terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL)-positive myonuclei were also found in Evans blue-stained muscle fibers but not in unstained ones. These results indicate that degenerating muscle fibers in the mdx mouse show an increase in membrane permeability and undergo apoptosis. Vital staining with Evans blue is useful not only for distinguishing degenerating muscle fibers, but also for studying the degeneration process biochemically in dystrophin-deficient muscle. This method may also be useful for evaluating the therapeutic effects of drug administration, gene transfer, and myoblast transfer in the mdx mouse.

Original languageEnglish
Pages (from-to)959-964
Number of pages6
JournalJournal of Biochemistry
Volume118
Issue number5
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Evans Blue
Dystrophin
Cell death
Muscle
Visualization
Apoptosis
Fibers
DNA
Mouse
Fiber
Gene transfer
DNA Nucleotidylexotransferase
Electrophoresis
Sepharose
Labeling

Keywords

  • Duchenne muscular dystrophy
  • Dystrophin
  • Evans blue
  • mdx mouse
  • Muscle degeneration

ASJC Scopus subject areas

  • Biochemistry
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology

Cite this

Visualization of dystrophic muscle fibers in Mdx mouse by vital staining with Evans blue : Evidence of apoptosis in dystrophin-deficient muscle. / Matsuda, Ryoichi; Nishikawa, Akio; Tanaka, Hideho.

In: Journal of Biochemistry, Vol. 118, No. 5, 1995, p. 959-964.

Research output: Contribution to journalArticle

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T1 - Visualization of dystrophic muscle fibers in Mdx mouse by vital staining with Evans blue

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