Abstract
Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.
| Original language | English |
|---|---|
| Pages (from-to) | 1674-1678 |
| Number of pages | 5 |
| Journal | Surface Science |
| Volume | 600 |
| Issue number | 8 |
| DOIs | |
| Publication status | Published - 2006 Apr 15 |
| Externally published | Yes |
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Keywords
- Atomic force microscopy
- Fibrinogen
- Highly oriented pyrolytic graphite
- Kelvin probe force microscopy
- Phase image
ASJC Scopus subject areas
- Physical and Theoretical Chemistry
- Condensed Matter Physics
- Surfaces and Interfaces
Cite this
Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy. / Ohta, Riichiro; Saito, Nagahiro; Ishizaki, Takahiro; Takai, Osamu.
In: Surface Science, Vol. 600, No. 8, 15.04.2006, p. 1674-1678.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy
AU - Ohta, Riichiro
AU - Saito, Nagahiro
AU - Ishizaki, Takahiro
AU - Takai, Osamu
PY - 2006/4/15
Y1 - 2006/4/15
N2 - Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.
AB - Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.
KW - Atomic force microscopy
KW - Fibrinogen
KW - Highly oriented pyrolytic graphite
KW - Kelvin probe force microscopy
KW - Phase image
UR - http://www.scopus.com/inward/record.url?scp=33646036367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646036367&partnerID=8YFLogxK
U2 - 10.1016/j.susc.2005.12.058
DO - 10.1016/j.susc.2005.12.058
M3 - Article
VL - 600
SP - 1674
EP - 1678
JO - Surface Science
JF - Surface Science
SN - 0039-6028
IS - 8
ER -