Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy

Riichiro Ohta, Nagahiro Saito, Takahiro Ishizaki, Osamu Takai

Research output: Contribution to journalArticle

28 Citations (Scopus)


Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.

Original languageEnglish
Pages (from-to)1674-1678
Number of pages5
JournalSurface Science
Issue number8
Publication statusPublished - 2006 Apr 15



  • Atomic force microscopy
  • Fibrinogen
  • Highly oriented pyrolytic graphite
  • Kelvin probe force microscopy
  • Phase image

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Surfaces, Coatings and Films
  • Materials Chemistry

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