Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy

Riichiro Ohta, Nagahiro Saito, Takahiro Ishizaki, Osamu Takai

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.

Original languageEnglish
Pages (from-to)1674-1678
Number of pages5
JournalSurface Science
Volume600
Issue number8
DOIs
Publication statusPublished - 2006 Apr 15
Externally publishedYes

Fingerprint

Plasma (human)
fibrinogen
Scanning probe microscopy
Graphite
pyrolytic graphite
Fibrinogen
Visualization
microscopy
scanning
probes
Microscopic examination
fibers
Fibers
Molecules
Surface potential
bundles
molecules
Atomic force microscopy
atomic force microscopy
air

Keywords

  • Atomic force microscopy
  • Fibrinogen
  • Highly oriented pyrolytic graphite
  • Kelvin probe force microscopy
  • Phase image

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces

Cite this

Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy. / Ohta, Riichiro; Saito, Nagahiro; Ishizaki, Takahiro; Takai, Osamu.

In: Surface Science, Vol. 600, No. 8, 15.04.2006, p. 1674-1678.

Research output: Contribution to journalArticle

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