Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy

Riichiro Ohta; Nagahiro Saito; Takahiro Ishizaki; Osamu Takai

doi:10.1016/j.susc.2005.12.058

Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy

Riichiro Ohta, Nagahiro Saito, Takahiro Ishizaki, Osamu Takai

Research output: Contribution to journal › Article › peer-review

28 Citations (Scopus)

Abstract

Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.

Original language	English
Pages (from-to)	1674-1678
Number of pages	5
Journal	Surface Science
Volume	600
Issue number	8
DOIs	https://doi.org/10.1016/j.susc.2005.12.058
Publication status	Published - 2006 Apr 15
Externally published	Yes

Keywords

Atomic force microscopy
Fibrinogen
Highly oriented pyrolytic graphite
Kelvin probe force microscopy
Phase image

ASJC Scopus subject areas

Condensed Matter Physics
Surfaces and Interfaces
Surfaces, Coatings and Films
Materials Chemistry

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title = "Visualization of human plasma fibrinogen adsorbed on highly oriented pyrolytic graphite by scanning probe microscopy",

abstract = "Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.",

keywords = "Atomic force microscopy, Fibrinogen, Highly oriented pyrolytic graphite, Kelvin probe force microscopy, Phase image",

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AU - Ohta, Riichiro

AU - Saito, Nagahiro

AU - Ishizaki, Takahiro

AU - Takai, Osamu

PY - 2006/4/15

Y1 - 2006/4/15

N2 - Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.

AB - Human plasma fibrinogen (HPF) was observed by atomic force microscopy (AFM) and Kelvin probe force microscopy (KPFM) conducted in non-contact mode. The HPF was adsorbed on a highly oriented pyrolytic graphite (HOPG) substrate as single molecules, as aggregated bundles, and as aggregated fibers. Topographic and phase images confirmed structural changes in the HPF after exposure to air, while topographic and KPFM images confirmed fibers with the width of a single HPF molecule. Additionally, KPFM confirmed the surface potential difference between the HPF and the HOPG, and periodical potential drop reflecting the E and D domains in the fiber.

KW - Atomic force microscopy

KW - Fibrinogen

KW - Highly oriented pyrolytic graphite

KW - Kelvin probe force microscopy

KW - Phase image

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