Wnt5a promotes adhesion of human dermal fibroblasts by triggering a phosphatidylinositol-3 kinase/Akt signal

Aya Kawasaki, Kosuke Torii, Yuki Yamashita, Koji Nishizawa, Kohsuke Kanekura, Masanori Katada, Minoru Ito, Ikuo Nishimoto, Kenzo Terashita, Sadakazu Aiso, Masaaki Matsuoka

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41 Citations (Scopus)

Abstract

Frizzled-3 (Fzd3), highly expressed in both the central nervous system (CNS) and skin, plays essential roles in axonal growth and guidance during the CNS development and may be involved in maintenance of skin integrity, although its ligand remains undetermined. In this study, we demonstrate that Wnt5a specifically binds to Fzd3 in vitro and triggers phosphorylation of Akt mediated by phosphatidylinositol-3 kinase (PI3K), but not that of ERK or protein kinase C, in human primary-cultured dermal fibroblasts. We have further found that such Wnt5a/Fzd3-triggered activation of the PI3K/Akt signal promotes integrin-mediated adhesion of human dermal fibroblasts to collagen I-coated dishes. Based on another finding that Wnt5a/Fzd3-triggered activation of the PI3K/Akt signal was blocked by an excess amount of a recombinant Fzd3-cysteine-rich domain (CRD), but not by that of a recombinant Fzd6-CRD, it is concluded that Wnt5a is a natural ligand of Fzd3 that triggers the PI3K/Akt signal and promotes adhesion of human dermal fibroblasts.

Original languageEnglish
Pages (from-to)2498-2506
Number of pages9
JournalCellular Signalling
Volume19
Issue number12
DOIs
Publication statusPublished - 2007 Dec 1

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Keywords

  • Adhesion
  • Frizzled
  • Frizzled-3
  • Fzd
  • Fzd3
  • Skin
  • Wnt
  • Wnt5a

ASJC Scopus subject areas

  • Cell Biology

Cite this

Kawasaki, A., Torii, K., Yamashita, Y., Nishizawa, K., Kanekura, K., Katada, M., Ito, M., Nishimoto, I., Terashita, K., Aiso, S., & Matsuoka, M. (2007). Wnt5a promotes adhesion of human dermal fibroblasts by triggering a phosphatidylinositol-3 kinase/Akt signal. Cellular Signalling, 19(12), 2498-2506. https://doi.org/10.1016/j.cellsig.2007.07.023