A bio-fluorometric acetone gas imaging system for the dynamic analysis of lipid metabolism in human breath

Takahiro Arakawa, Naoki Mizukoshi, Kenta Iitani, Koji Toma, Kohji Mitsubayashi

研究成果: Article査読

1 被引用数 (Scopus)

抄録

We constructed an imaging system to measure the concentration of acetone gas by acetone reduction using secondary alcohol dehydrogenase (S-ADH). Reduced nicotinamide adenine dinucleotide (NADH) was used as an electron donor, and acetone was imaged by fluorescence detection of the decrease in the autofluorescence of NADH. In this system, S-ADH–immobilized membranes wetted with buffer solution containing NADH were placed in a dark box, and UV-LED excitation sheets and a high-sensitivity camera were installed on both sides of the optical axis to enable loading of acetone gas. A hydrophilic polytetrafluoroethylene (H-PTFE) membrane with low autofluorescence was used as a substrate, and honeycomb-like through-hole structures were fabricated using a CO2 laser device. After loading the enzyme membrane with acetone gas standards, a decrease in fluorescence intensity was observed in accordance with the concentration of acetone gas. The degree of decrease in fluorescence intensity was calculated using image analysis software; it was possible to quantify acetone gas at concentrations of 50–2000 ppb, a range that includes the exhaled breath concentration of acetone in healthy subjects. We applied this imaging system to measure the acetone gas in the air exhaled by a healthy individual during fasting.

本文言語English
論文番号258
ジャーナルChemosensors
9
9
DOI
出版ステータスPublished - 2021 9月
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 物理化学および理論化学

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