A Gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion

Yoshitaka Nakayama; Kenjiro Yoshimura; Hidetoshi Iida

doi:10.1128/AEM.01310-12

A Gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion

Yoshitaka Nakayama, Kenjiro Yoshimura, Hidetoshi Iida

研究成果: Comment/debate › 査読

24 被引用数 (Scopus)

抄録

The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.

本文言語	English
ページ（範囲）	5432-5434
ページ数	3
ジャーナル	Applied and Environmental Microbiology
巻	78
号	15
DOI	https://doi.org/10.1128/AEM.01310-12
出版ステータス	Published - 2012 8月
外部発表	はい

ASJC Scopus subject areas

バイオテクノロジー
食品科学
応用微生物学とバイオテクノロジー
生態学

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10.1128/AEM.01310-12

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abstract = "The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.",

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AU - Iida, Hidetoshi

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AB - The A-to-V mutation at position 111 (A111V) in the mechanosensitive channel NCgl1221 (MscCG) causes constitutive glutamate secretion in Corynebacterium glutamicum. Patch clamp experiments revealed that NCgl1221 (A111V) had a significantly smaller gating threshold than the wild-type counterpart and displayed strong hysteresis, suggesting that the gain-of-function mutation in the gating of NCgl1221 leads to the oversecretion of glutamate.

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JO - Applied and Environmental Microbiology

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A Gain-of-function mutation in gating of Corynebacterium glutamicum NCgl1221 causes constitutive glutamate secretion

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