A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets

Keita Hamasaki, Robert R. Rando

研究成果: Article

39 引用 (Scopus)

抄録

Since RNA molecules can form intricate three-dimensional structures, it should be possible to design specific, high-affinity antagonists directed against these structures. To begin to explore the validity of this possibility, high-throughput screening methods are required to assay for RNA antagonists. A fluorescence quenching technique is described here in a 96- well plate format which is capable of screening chemical diversity libraries. A pyrene-containing aminoglycoside analog is used to accurately monitor antagonist binding to a prokaryotic 16S rRNA A-site decoding region construct. This rRNA region comprises the natural target for aminoglycoside antibiotics. The fluorescence technique reported here should be generally adaptable to monitor the binding of structurally novel antagonists to any selected RNA target.

元の言語English
ページ(範囲)183-190
ページ数8
ジャーナルAnalytical Biochemistry
261
発行部数2
DOI
出版物ステータスPublished - 1998 8 1
外部発表Yes

Fingerprint

Fluorescence
Throughput
Aminoglycosides
RNA
Screening
Decoding
Quenching
Assays
Anti-Bacterial Agents
Molecules
pyrene

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets. / Hamasaki, Keita; Rando, Robert R.

:: Analytical Biochemistry, 巻 261, 番号 2, 01.08.1998, p. 183-190.

研究成果: Article

@article{bd0239f224734859b829d8e2995b7e81,
title = "A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets",
abstract = "Since RNA molecules can form intricate three-dimensional structures, it should be possible to design specific, high-affinity antagonists directed against these structures. To begin to explore the validity of this possibility, high-throughput screening methods are required to assay for RNA antagonists. A fluorescence quenching technique is described here in a 96- well plate format which is capable of screening chemical diversity libraries. A pyrene-containing aminoglycoside analog is used to accurately monitor antagonist binding to a prokaryotic 16S rRNA A-site decoding region construct. This rRNA region comprises the natural target for aminoglycoside antibiotics. The fluorescence technique reported here should be generally adaptable to monitor the binding of structurally novel antagonists to any selected RNA target.",
author = "Keita Hamasaki and Rando, {Robert R.}",
year = "1998",
month = "8",
day = "1",
doi = "10.1006/abio.1998.2740",
language = "English",
volume = "261",
pages = "183--190",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets

AU - Hamasaki, Keita

AU - Rando, Robert R.

PY - 1998/8/1

Y1 - 1998/8/1

N2 - Since RNA molecules can form intricate three-dimensional structures, it should be possible to design specific, high-affinity antagonists directed against these structures. To begin to explore the validity of this possibility, high-throughput screening methods are required to assay for RNA antagonists. A fluorescence quenching technique is described here in a 96- well plate format which is capable of screening chemical diversity libraries. A pyrene-containing aminoglycoside analog is used to accurately monitor antagonist binding to a prokaryotic 16S rRNA A-site decoding region construct. This rRNA region comprises the natural target for aminoglycoside antibiotics. The fluorescence technique reported here should be generally adaptable to monitor the binding of structurally novel antagonists to any selected RNA target.

AB - Since RNA molecules can form intricate three-dimensional structures, it should be possible to design specific, high-affinity antagonists directed against these structures. To begin to explore the validity of this possibility, high-throughput screening methods are required to assay for RNA antagonists. A fluorescence quenching technique is described here in a 96- well plate format which is capable of screening chemical diversity libraries. A pyrene-containing aminoglycoside analog is used to accurately monitor antagonist binding to a prokaryotic 16S rRNA A-site decoding region construct. This rRNA region comprises the natural target for aminoglycoside antibiotics. The fluorescence technique reported here should be generally adaptable to monitor the binding of structurally novel antagonists to any selected RNA target.

UR - http://www.scopus.com/inward/record.url?scp=0032144561&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032144561&partnerID=8YFLogxK

U2 - 10.1006/abio.1998.2740

DO - 10.1006/abio.1998.2740

M3 - Article

C2 - 9716420

AN - SCOPUS:0032144561

VL - 261

SP - 183

EP - 190

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 2

ER -