A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets

Keita Hamasaki, Robert R. Rando

研究成果査読

41 被引用数 (Scopus)

抄録

Since RNA molecules can form intricate three-dimensional structures, it should be possible to design specific, high-affinity antagonists directed against these structures. To begin to explore the validity of this possibility, high-throughput screening methods are required to assay for RNA antagonists. A fluorescence quenching technique is described here in a 96- well plate format which is capable of screening chemical diversity libraries. A pyrene-containing aminoglycoside analog is used to accurately monitor antagonist binding to a prokaryotic 16S rRNA A-site decoding region construct. This rRNA region comprises the natural target for aminoglycoside antibiotics. The fluorescence technique reported here should be generally adaptable to monitor the binding of structurally novel antagonists to any selected RNA target.

本文言語English
ページ(範囲)183-190
ページ数8
ジャーナルAnalytical Biochemistry
261
2
DOI
出版ステータスPublished - 1998 8 1
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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