A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets

Keita Hamasaki, Robert R. Rando

研究成果: Article

40 引用 (Scopus)

抜粋

Since RNA molecules can form intricate three-dimensional structures, it should be possible to design specific, high-affinity antagonists directed against these structures. To begin to explore the validity of this possibility, high-throughput screening methods are required to assay for RNA antagonists. A fluorescence quenching technique is described here in a 96- well plate format which is capable of screening chemical diversity libraries. A pyrene-containing aminoglycoside analog is used to accurately monitor antagonist binding to a prokaryotic 16S rRNA A-site decoding region construct. This rRNA region comprises the natural target for aminoglycoside antibiotics. The fluorescence technique reported here should be generally adaptable to monitor the binding of structurally novel antagonists to any selected RNA target.

元の言語English
ページ(範囲)183-190
ページ数8
ジャーナルAnalytical Biochemistry
261
発行部数2
DOI
出版物ステータスPublished - 1998 8 1
外部発表Yes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

フィンガープリント A high-throughput fluorescence screen to monitor the specific binding of antagonists to RNA targets' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用