A high-throughput screening utilization intramolecular fluorescence resonance energy transfer for the discovery of the molecules that bind HIV-1 TAR RNA specifically

Chiharu Matsumoto, Keita Hamasaki, Hisakazu Mihara, Akihiko Ueno

研究成果: Article査読

54 被引用数 (Scopus)

抄録

A 16-residue peptide, including the Tat49-57 sequence was labeled with a fluorescein and a tetramethylrhodamine at its N- and C-terminus, respectively. This double dye-labeled peptide was prepared as a tracer for high-throughput screening utilizing intramolecular fluorescence resonance energy transfer (FRET). The binding of the competitor molecules for HIV-1 TAR RNA were monitored and dissociation constants of those molecule were determined by using this tracer. This novel screening system might be useful to discover the drug for HIV-1 TAR RNA. (C) 2000 Elsevier Science Ltd. All rights reserved.

本文言語English
ページ(範囲)1857-1861
ページ数5
ジャーナルBioorganic and Medicinal Chemistry Letters
10
16
DOI
出版ステータスPublished - 2000 8月 21
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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