A monoclonal antibody against human kidney gamma-glutamyl transpeptidase: Preparation, immunochemical, and immunohistochemical characterization

M. Shiozawa, S. Yamashita, S. Aiso, K. Yasuda

研究成果: Article査読

22 被引用数 (Scopus)

抄録

To perform immunohistochemical study of the distribution of γ-glutamyl transpeptidase in human organs, a highly specific antibody against the human enzyme is required. We prepared monoclonal antibody against γ-glutamyl transpeptidase from human kidney, using the hybridoma technique. The antibody was of the IgG1 type and the light chain belonged to the κ class. The antibody reacted specifically with the 63 KD heavy subunit of the enzyme. Examination of the specificity of the antibody performed by immunohistochemical staining of human kidney sections revealed that the antigen was localized on the brush border and along the basolateral membrane of the epithelial cells of both the convoluted and the straight portions of the proximal tubule. This antibody was also reactive in several human organs other than kidney, including epididymis, prostate, seminal vesicle, pancreas, and normal liver, and in human hepatoma. These findings indicate the existence of an antigenic determinant common to human kidney and other organs. The monoclonal antibody did not crossreact with mouse, rat, guinea pig, rabbit, or pig kidney.

本文言語English
ページ(範囲)1053-1061
ページ数9
ジャーナルJournal of Histochemistry and Cytochemistry
37
7
DOI
出版ステータスPublished - 1989
外部発表はい

ASJC Scopus subject areas

  • 解剖学
  • 組織学

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