A novel Xenopus laevis SRY-related gene, xSox33

Jun Hagiuda, Yoshiki Hiraoka, Masanori Hasegawa, Motoyuki Ogawa, Sadakazu Aiso

研究成果: Article査読

2 被引用数 (Scopus)

抄録

The sex-determining region Y (SRY) gene and its related Sox genes encode transcriptional regulatory factors. In this study, we isolated and sequenced a novel Sox cDNA from African clawed frog (Xenopus laevis). The Sox gene was named xSox33. xSox33 was revealed to encode 244 amino acids. Reverse transcription-polymerase chain reaction (RT-PCR) showed that xSox33 was expressed at very low levels in some frog tissues including lung, ovary, skeletal muscle, testis, brain and heart. Its embryonic expression was also studied by RT-PCR. After the mid-blastula transition, the zygotic expression was initiated during gastrulation and the level was elevated as the embryogenesis proceeded. Electrophoretic mobility shift assay (EMSA) indicated that a recombinant xSox33 polypeptide was capable of binding to the nucleotide sequence AACAAT.

本文言語English
ページ(範囲)140-145
ページ数6
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1628
2
DOI
出版ステータスPublished - 2003 7 28
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

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