A photoresponsive polymer surface which enables in situ control of living cell adhesion

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Katsuhide Ohi, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

研究成果: Conference contribution

抄録

Novel photoresponsive culture surface (PRCS) that can control cell adhesion by photo-irradiation and temperature change was prepared with polymethylmethacrylate and a photoresponsive polymer containing photochromic chromophore, 6-nitrospiropyran. On the PRCS, CHO-K1 cells were attached evenly after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) for 5 min and cooled in fresh medium at 15°C for 15 min. Then the PRCS was washed by fresh medium at 15°C. In the washing process, the adhering cells were immobilized in the irradiated region, while the cells were easily detached in the other region. Adhering cells were confirmed as alive because they were stained by CMTPX reagent. As a result, living cell patterning with the 100 μm line width was produced.

元の言語English
ホスト出版物のタイトルPolymer Preprints, Japan
ページ2339
ページ数1
54
エディション1
出版物ステータスPublished - 2005
イベント54th SPSJ Annual Meeting 2005 - Yokohama
継続期間: 2005 5 252005 5 27

Other

Other54th SPSJ Annual Meeting 2005
Yokohama
期間05/5/2505/5/27

Fingerprint

Cell adhesion
Polymers
Chromophores
Cell culture
Washing
Linewidth
Cells
Irradiation
Temperature

ASJC Scopus subject areas

  • Engineering(all)

これを引用

Edahiro, J. I., Sumaru, K., Tada, Y., Ohi, K., Takagi, T., Shinbo, T., ... Yoshimi, Y. (2005). A photoresponsive polymer surface which enables in situ control of living cell adhesion. : Polymer Preprints, Japan (1 版, 巻 54, pp. 2339)

A photoresponsive polymer surface which enables in situ control of living cell adhesion. / Edahiro, Jun Ichi; Sumaru, Kimio; Tada, Yuichi; Ohi, Katsuhide; Takagi, Toshiyuki; Shinbo, Toshio; Kanamori, Toshiyuki; Yoshimi, Yasuo.

Polymer Preprints, Japan. 巻 54 1. 編 2005. p. 2339.

研究成果: Conference contribution

Edahiro, JI, Sumaru, K, Tada, Y, Ohi, K, Takagi, T, Shinbo, T, Kanamori, T & Yoshimi, Y 2005, A photoresponsive polymer surface which enables in situ control of living cell adhesion. : Polymer Preprints, Japan. 1 Edn, 巻. 54, pp. 2339, 54th SPSJ Annual Meeting 2005, Yokohama, 05/5/25.
Edahiro JI, Sumaru K, Tada Y, Ohi K, Takagi T, Shinbo T その他. A photoresponsive polymer surface which enables in situ control of living cell adhesion. : Polymer Preprints, Japan. 1 版 巻 54. 2005. p. 2339
Edahiro, Jun Ichi ; Sumaru, Kimio ; Tada, Yuichi ; Ohi, Katsuhide ; Takagi, Toshiyuki ; Shinbo, Toshio ; Kanamori, Toshiyuki ; Yoshimi, Yasuo. / A photoresponsive polymer surface which enables in situ control of living cell adhesion. Polymer Preprints, Japan. 巻 54 1. 版 2005. pp. 2339
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AU - Edahiro, Jun Ichi

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AU - Tada, Yuichi

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AU - Takagi, Toshiyuki

AU - Shinbo, Toshio

AU - Kanamori, Toshiyuki

AU - Yoshimi, Yasuo

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