A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

研究成果: Conference contribution

抄録

We have been developing a novel "on-demand cell manipulation technique" which enables control of cell adhesion by regional photo-irradiation on adhering region of objective cells. In this study, our objective is to improve performance of the "photoresponsive culture surface (PRCS) " that can control cell adhesion by photo-irradiation and temperature change and that is the key material for our novel technique. Firstly, cell adhesion control of extended cells was successfully achieved for the first time. PRCS was prepared with pory(N-isopropylacrylamide) containing photochromic chromophore, 6-nitrospiropyran. On this PRCS, MDCK cells were attached and extended evenly as a sheet after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) and cooled at 15°C. Then the PRCS was washed by fresh medium to remove weakly adhering cells in non-irradiated region. As a result, we confirmed that this method was applicable even to extended cells. Additionally, co-culture system was produced in simple procedures by culturing another kind of cells (CHO-K1 cells) in the cell-detached area. Secondary, for lowering cell damage from low temperature treatment, we prepared another type of PRCS with poly(N,N-dimethylacrylamide) containing 6-nitrospiropyran, which has no thermoresponsive nature. We confirmed that adhesion of MDCK cells on this novel PRCS could be changed only by photo-irradiation without any temperature change. Additionally, this result also supports our hypothesis that spiropyran chromophore directly captures cells in cell adhesion control.

元の言語English
ホスト出版物のタイトルPolymer Preprints, Japan
ページ5213-5214
ページ数2
54
エディション2
出版物ステータスPublished - 2005
イベント54th SPSJ Symposium on Macromolecules - Yamagata
継続期間: 2005 9 202005 9 22

Other

Other54th SPSJ Symposium on Macromolecules
Yamagata
期間05/9/2005/9/22

Fingerprint

Adhesives
Cell adhesion
Polymers
Irradiation
Chromophores
Cell culture
Temperature
Adhesion
Cells

ASJC Scopus subject areas

  • Engineering(all)

これを引用

Edahiro, J. I., Sumaru, K., Tada, Y., Takagi, T., Shinbo, T., Kanamori, T., & Yoshimi, Y. (2005). A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. : Polymer Preprints, Japan (2 版, 巻 54, pp. 5213-5214)

A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. / Edahiro, Jun Ichi; Sumaru, Kimio; Tada, Yuichi; Takagi, Toshiyuki; Shinbo, Toshio; Kanamori, Toshiyuki; Yoshimi, Yasuo.

Polymer Preprints, Japan. 巻 54 2. 編 2005. p. 5213-5214.

研究成果: Conference contribution

Edahiro, JI, Sumaru, K, Tada, Y, Takagi, T, Shinbo, T, Kanamori, T & Yoshimi, Y 2005, A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. : Polymer Preprints, Japan. 2 Edn, 巻. 54, pp. 5213-5214, 54th SPSJ Symposium on Macromolecules, Yamagata, 05/9/20.
Edahiro JI, Sumaru K, Tada Y, Takagi T, Shinbo T, Kanamori T その他. A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. : Polymer Preprints, Japan. 2 版 巻 54. 2005. p. 5213-5214
Edahiro, Jun Ichi ; Sumaru, Kimio ; Tada, Yuichi ; Takagi, Toshiyuki ; Shinbo, Toshio ; Kanamori, Toshiyuki ; Yoshimi, Yasuo. / A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. Polymer Preprints, Japan. 巻 54 2. 版 2005. pp. 5213-5214
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AU - Kanamori, Toshiyuki

AU - Yoshimi, Yasuo

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