A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells

Jun Ichi Edahiro, Kimio Sumaru, Yuichi Tada, Toshiyuki Takagi, Toshio Shinbo, Toshiyuki Kanamori, Yasuo Yoshimi

研究成果: Paper

抜粋

We have been developing a novel "on-demand cell manipulation technique" which enables control of cell adhesion by regional photo-irradiation on adhering region of objective cells. In this study, our objective is to improve performance of the "photoresponsive culture surface (PRCS) " that can control cell adhesion by photo-irradiation and temperature change and that is the key material for our novel technique. Firstly, cell adhesion control of extended cells was successfully achieved for the first time. PRCS was prepared with pory(N-isopropylacrylamide) containing photochromic chromophore, 6-nitrospiropyran. On this PRCS, MDCK cells were attached and extended evenly as a sheet after incubation at 37°C. The PRCS was regionally irradiated with light (365 nm) and cooled at 15°C. Then the PRCS was washed by fresh medium to remove weakly adhering cells in non-irradiated region. As a result, we confirmed that this method was applicable even to extended cells. Additionally, co-culture system was produced in simple procedures by culturing another kind of cells (CHO-K1 cells) in the cell-detached area. Secondary, for lowering cell damage from low temperature treatment, we prepared another type of PRCS with poly(N,N-dimethylacrylamide) containing 6-nitrospiropyran, which has no thermoresponsive nature. We confirmed that adhesion of MDCK cells on this novel PRCS could be changed only by photo-irradiation without any temperature change. Additionally, this result also supports our hypothesis that spiropyran chromophore directly captures cells in cell adhesion control.

元の言語English
ページ5213-5214
ページ数2
出版物ステータスPublished - 2005 12 1
イベント54th SPSJ Symposium on Macromolecules - Yamagata, Japan
継続期間: 2005 9 202005 9 22

Conference

Conference54th SPSJ Symposium on Macromolecules
Japan
Yamagata
期間05/9/2005/9/22

ASJC Scopus subject areas

  • Engineering(all)

フィンガープリント A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Edahiro, J. I., Sumaru, K., Tada, Y., Takagi, T., Shinbo, T., Kanamori, T., & Yoshimi, Y. (2005). A photoresponsive polymer surface which enables on-demand manipulation of adhesive cells. 5213-5214. 論文発表場所 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.