A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel

Tomohisa Ogawa, Nirai Matsunaga, Saori Inomata, Masato Tanaka, Nobuyuki Futai

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We have developed a cast microfluidic chip that contains a thin (∼2 μm wide) microchannel that is smoothly connected to thick microfluidics. The thin line features having high aspect ratio for a low-cost photolithography in which an emulsion photomask was used (1-1 ∼ 1-3) were fabricated by exposing SU-8 photoresist to diffused 185 nm UV light emitted by a low-cost ozone lamp from the backside of the substrate to ensure sufficient crosslinking of small regions of the SU-8 photoresist. An H-shaped microfluidic configuration was used, in which the thin channel maintained constant diffusion fronts beyond purely static diffusion. We also demonstrated the long-term effects of a gradient of nerve growth factor on axon elongation by primary neurons cultured in the micro channel.

本文言語English
ホスト出版物のタイトル2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
ページ5521-5524
ページ数4
DOI
出版ステータスPublished - 2013 10 31
イベント2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka, Japan
継続期間: 2013 7 32013 7 7

出版物シリーズ

名前Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN(印刷版)1557-170X

Conference

Conference2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
国/地域Japan
CityOsaka
Period13/7/313/7/7

ASJC Scopus subject areas

  • 信号処理
  • 生体医工学
  • コンピュータ ビジョンおよびパターン認識
  • 健康情報学

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