A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel

Tomohisa Ogawa, Nirai Matsunaga, Saori Inomata, Masato Tanaka, Nobuyuki Futai

研究成果: Conference contribution

2 引用 (Scopus)

抄録

We have developed a cast microfluidic chip that contains a thin (∼2 μm wide) microchannel that is smoothly connected to thick microfluidics. The thin line features having high aspect ratio for a low-cost photolithography in which an emulsion photomask was used (1-1 ∼ 1-3) were fabricated by exposing SU-8 photoresist to diffused 185 nm UV light emitted by a low-cost ozone lamp from the backside of the substrate to ensure sufficient crosslinking of small regions of the SU-8 photoresist. An H-shaped microfluidic configuration was used, in which the thin channel maintained constant diffusion fronts beyond purely static diffusion. We also demonstrated the long-term effects of a gradient of nerve growth factor on axon elongation by primary neurons cultured in the micro channel.

元の言語English
ホスト出版物のタイトルProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ページ5521-5524
ページ数4
DOI
出版物ステータスPublished - 2013
イベント2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013 - Osaka
継続期間: 2013 7 32013 7 7

Other

Other2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013
Osaka
期間13/7/313/7/7

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Microchannels
Microfluidics
Aspect ratio
Photoresists
Photomasks
Photolithography
Electric lamps
Ultraviolet radiation
Crosslinking
Ozone
Neurons
Emulsions
Costs
Elongation
Substrates

ASJC Scopus subject areas

  • Computer Vision and Pattern Recognition
  • Signal Processing
  • Biomedical Engineering
  • Health Informatics

これを引用

Ogawa, T., Matsunaga, N., Inomata, S., Tanaka, M., & Futai, N. (2013). A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. : Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS (pp. 5521-5524). [6610800] https://doi.org/10.1109/EMBC.2013.6610800

A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. / Ogawa, Tomohisa; Matsunaga, Nirai; Inomata, Saori; Tanaka, Masato; Futai, Nobuyuki.

Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 5521-5524 6610800.

研究成果: Conference contribution

Ogawa, T, Matsunaga, N, Inomata, S, Tanaka, M & Futai, N 2013, A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. : Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS., 6610800, pp. 5521-5524, 2013 35th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2013, Osaka, 13/7/3. https://doi.org/10.1109/EMBC.2013.6610800
Ogawa T, Matsunaga N, Inomata S, Tanaka M, Futai N. A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. : Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. p. 5521-5524. 6610800 https://doi.org/10.1109/EMBC.2013.6610800
Ogawa, Tomohisa ; Matsunaga, Nirai ; Inomata, Saori ; Tanaka, Masato ; Futai, Nobuyuki. / A simple microfluidic gradient generator with a soft-lithographically prototyped, high-aspect-ratio, ∼2 μm wide microchannel. Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS. 2013. pp. 5521-5524
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