Decellularized tissues, which are extracellular matrix assembly prepared from living tissue, are tried to apply for regenerative medicine and to be used as biomaterials because of the good biocompatibility. Recently, many researchers are challenging to resolve its mechanism, but it is still unclear. Previously, our group also reported that decellularized tissues were prepared by high hydrostatic pressure (HHP) technology and they showed good biocompatibility . Especially, decellularized aorta showed good patency and early endothelialization for in vivo vascular replacement .One possible reason is that HHP decellularized luminal surface remains the similar morphology to native aortic lumenal surface. In this study, we investigated the surface morphology of aortic lumen prepared by various decellularization methods using HHP and SDS, and synthetic replicas were prepared by a transferring method using poly(dimethyl siloxane) (PDMS). The endothelial cell behavior on them was investigated (Figure 1).