Styrene, allylamine, and acrylic acid were polymerized on a quartz crystal microbalance (QCM) by plasma polymerization technique under 100 W, 100 P, and a settled period of each. Coating amounts of plasma polymerized films (pp-films) were determined by QCM technique. Adsorption amounts of anti-C-reactive protein monoclonal IgG antibody (CRP antibody) and its F(ab')2 fragment of IgG antibody (CRP-F(ab′)2 antibody) on three plasma-polymerized films and on a gold QCM surface as a reference were also determined by QCM technique. Immunoreaction amounts between antigen (CRP) and antibodies adsorbed QCM on pp-films were determined by QCM. Adsorption amount of CRP antibodies or CRP-F(ab′)2 antibodies for pp-films and gold were almost similar levels in the range of 50 to 100 Hz per area. Immunoreaction amounts of CRP depended on the combination of pp-films and antibodies. The largest dose-responses of immunoreaction were obtained for pp-acrylic acid for CRP antibodies, and pp-allylamine for CRP-F(ab′)2 antibodies. These results imply that molecular orientation of antibodies adsorbed on pp-films may be performed in different manners.
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