All-in-one uniified microfluidic chip for automation of embryonic cell manipulation

Shinya Sakuma, Yoko Yamanishi, Fumihito Arai, Tatsuo Arai, Akiyuki Hasegawa, Tamio Tanikawa, Akihiko Ichikawa, Osamu Satoh, Akihiro Nakayama, Hiroshi Aso, Mitsuhiro Goto, Seiya Takahashi, Kazutsugu Matsukawa

研究成果: Conference contribution

4 被引用数 (Scopus)

抄録

We developed a microfluidic chip for automation of cloning process based on a new protocol. The protocol is based on removal of the zona pellucida outside the chip which contributes to simplify on-chip automation of cloning. Then, the oocytes are put into the chip. The design concept of the chip is summarized as follows. (1) The oocyte is cut into two parts. (2) The divided half oocyte is sorted with and without nucleus. (3) The half oocyte without nucleus is coupled with a donor cell, and they are fused by an electrical field. For the current study, the microfluidic chip was designed to have 3 parts for this process. Then, all-in-one unified microfluidic chip was successfully fabricated. Basic functions of these parts are verified independently.

本文言語English
ホスト出版物のタイトルProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社Chemical and Biological Microsystems Society
ページ1883-1885
ページ数3
ISBN(印刷版)9780979806421
出版ステータスPublished - 2009
イベント13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
継続期間: 2009 11 12009 11 5

出版物シリーズ

名前Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
国/地域Korea, Republic of
CityJeju
Period09/11/109/11/5

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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