AlphaV beta3 (αvβ3) integrin inhibition reduces leukocyte-endothelium interaction in a pressure-induced reperfusion model

Shigeru Ichioka, Naomi Sekiya, Masahiro Shibata, Takashi Nakatsuka

研究成果: Article査読

6 被引用数 (Scopus)

抄録

The leukocyte-endothelium interaction is known to contribute to reperfusion injury, which is considered to participate in the pathophysiology of pressure ulcers, and integrin alphaV beta3 (αvβ3) has been shown to mediate the processes of cellular adhesion in various types of cells. This study aims to clarify leukocyte behavior in our original microcirculatory pressure-induced reperfusion model, which can visualize the microcirculation in vivo. We also estimated the effect of αvβ3 integrin inhibition on the reduction of the leukocyte-endothelium interaction. Mice with dorsal skinfold chambers were divided into three groups: the baseline group (n=6), in which animals received no compression; the compression-reperfusion group (n=6), in which animals underwent 2-hour compression of the dorsal skin, followed by release, and the inhibitor-treated group (n=7), in which an αvβ3 inhibitor, CP4715, was administered in addition to the compression-release procedure. Staining with rhodamine 6G quantitatively visualized leukocyte behavior under the intravital fluorescent microscope. Compression-reperfusion induced a significant increase in rolling, sticking, and extravasation of the leukocytes. Treatment with the inhibitor strikingly reduced leukocyte sticking and extravasation. The present experiment has provided evidence that αvβ3 inhibition reduces leukocyte-endothelium interaction in our original pressure-induced reperfusion model.

本文言語English
ページ(範囲)572-576
ページ数5
ジャーナルWound Repair and Regeneration
15
4
DOI
出版ステータスPublished - 2007 7月

ASJC Scopus subject areas

  • 外科
  • 皮膚病学

フィンガープリント

「AlphaV beta3 (αvβ3) integrin inhibition reduces leukocyte-endothelium interaction in a pressure-induced reperfusion model」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル