Alteration of substrate specificity of cholesterol oxidase from Streptomyces sp. by site-directed mutagenesis

Engineering & Materials Science

• Amino acids 19%
• Catalyst activity 13%
• Cholesterol 90%
• Enzyme activity 8%
• Enzymes 67%
• Genes 5%
• Mutagenesis 92%
• Oxidoreductases 88%
• Substitution reactions 20%
• Substrates 41%
• Temperature 2%

Medicine & Life Sciences

• Amino Acid Substitution 10%
• Amino Acids 3%
• Brevibacterium 16%
• Cholesterol 10%
• Cholesterol Oxidase 100%
• Enzymes 32%
• Genes 1%
• Mutation 2%
• Pregnenolone 20%
• Serum 2%
• Site-Directed Mutagenesis 62%
• Sterols 5%
• Streptomyces 72%
• Substrate Specificity 65%
• Temperature 3%
• Viperidae 6%

Chemical Compounds

• Acid 5%
• Amino-Acid Residue 7%
• Cholesterol 52%
• Kcat/Km 10%
• Mutation 7%
• pH Value 3%
• Probe 5%
• Site-Directed Mutagenesis 60%
• Sterol 9%
• Substitution Reaction 21%