Altered cellular responses to serum mitogens, including platelet‐derived growth factor, in cultured smooth muscle cells derived from arteries of patients with moyamoya disease

Masaru Aoyagi, Naomi Fukai, Hiroshi Sakamoto, Tadashi Shinkai, Yoshiharu Matsushima, Mari Yamamoto, Kiyotaka Yamamoto

研究成果: Article査読

63 被引用数 (Scopus)

抄録

Progressive stenosis or occlusion of bi ateral internal carotid arteries by fibrocellular intimal thickening results in cerebral ischemia in moyamoya disease. The etiology is unknown. We examined cultured arterial smooth muscle cells (SMC) from scalp arteries of five patients with moyamoya disease. In this study we investigated the responsiveness of the cells in culture to serum mitogens including platelet‐derived growth factor (PDGF), a major mitogen of SMC, and compared the response to that of cells derived from age‐matched control patients. SMC from patients with moyamoya disease proliferated less rapidly in a medium with 15% serum than did control SMC and responded poorly to the addition of PDGF to 5% serum. PDGF alone did not stimulate SMC in a quiescent state to initiate DNA synthesis in moyamoya disease, without serum factors other than bovine serum albumin, though it significantly stimulated the controls. Simultaneous additions of epidermal growth factor, insulin‐like growth factor‐I, and PDGF stimulated initiation of DNA synthesis in cells from moyamoya disease, but not as much as PDGF alone did in the controls. Although direct correlations with the pathogenesis of the disease remain to be clarified, the results indicate altered interrelations between serum factors and the cellular responses in vessels of moyamoya disease.

本文言語English
ページ(範囲)191-198
ページ数8
ジャーナルJournal of Cellular Physiology
147
2
DOI
出版ステータスPublished - 1991 5月

ASJC Scopus subject areas

  • 生理学
  • 臨床生化学
  • 細胞生物学

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