Amperometric biosensor based on carbon nanotube functionalized by redox plasma-polymerized film

Tatsuya Hoshino, Hitoshi Muguruma

研究成果: Article

1 引用 (Scopus)

抄録

A novel fabrication approach for the amperometric biosensor based on multilayer films containing carbon nanotubes (CNT), a plasma-polymerized film (PPF), and enzyme glucose oxidase (GOD) is reported. The configuration of the electrochemical electrode is sequentially composed of sputtered gold, lower acetonitrile PPF, CNT, redox PPF, GOD, and upper acetonitrile PPF (denoted as PPF/GOD/Redox-PPF/CNT/PPF/Au). The lower acetonitrile PPF deposited on Au acts as a permselective membrane, and as a scaffold for CNT layer formation. The upper acetonirile PPF directly deposited on GOD acts as a matrix for enzyme immobilization. The redox PPF polymerized by a monomer of dimethlyaminomethlyferrocene (DAF) is directly deposited onto CNTs. The surface of the functionalized CNT has redox sites of ferrocene groups that shuttle electrons from CNTs to the sensing surface of the Au electrode. The synergy between the redox PPF and CNT provides benefits in terms of lowering the operational potential and enhancing the sensitivity (current). The optimized glucose biosensor revealed a sensitivity of 2.0μAmM-1 cm -2 at +0.4 V vs Ag/AgCl, a linear dynamic range of 4.9-27mM, and a response time of 5 s.

元の言語English
記事番号085202
ジャーナルJapanese Journal of Applied Physics
50
発行部数8 PART 1
DOI
出版物ステータスPublished - 2011 8

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bioinstrumentation
Biosensors
Carbon nanotubes
carbon nanotubes
Plasmas
Glucose oxidase
glucose
oxidase
Acetonitrile
acetonitrile
Oxidation-Reduction
enzymes
Permselective membranes
Electrochemical electrodes
Enzyme immobilization
Multilayer films
electrodes
sensitivity
Scaffolds
immobilization

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy(all)

これを引用

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abstract = "A novel fabrication approach for the amperometric biosensor based on multilayer films containing carbon nanotubes (CNT), a plasma-polymerized film (PPF), and enzyme glucose oxidase (GOD) is reported. The configuration of the electrochemical electrode is sequentially composed of sputtered gold, lower acetonitrile PPF, CNT, redox PPF, GOD, and upper acetonitrile PPF (denoted as PPF/GOD/Redox-PPF/CNT/PPF/Au). The lower acetonitrile PPF deposited on Au acts as a permselective membrane, and as a scaffold for CNT layer formation. The upper acetonirile PPF directly deposited on GOD acts as a matrix for enzyme immobilization. The redox PPF polymerized by a monomer of dimethlyaminomethlyferrocene (DAF) is directly deposited onto CNTs. The surface of the functionalized CNT has redox sites of ferrocene groups that shuttle electrons from CNTs to the sensing surface of the Au electrode. The synergy between the redox PPF and CNT provides benefits in terms of lowering the operational potential and enhancing the sensitivity (current). The optimized glucose biosensor revealed a sensitivity of 2.0μAmM-1 cm -2 at +0.4 V vs Ag/AgCl, a linear dynamic range of 4.9-27mM, and a response time of 5 s.",
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