抄録
A novel fabrication approach for the amperometric biosensor based on multilayer films containing carbon nanotubes (CNT), a plasma-polymerized film (PPF), and enzyme glucose oxidase (GOD) is reported. The configuration of the electrochemical electrode is sequentially composed of sputtered gold, lower acetonitrile PPF, CNT, redox PPF, GOD, and upper acetonitrile PPF (denoted as PPF/GOD/Redox-PPF/CNT/PPF/Au). The lower acetonitrile PPF deposited on Au acts as a permselective membrane, and as a scaffold for CNT layer formation. The upper acetonirile PPF directly deposited on GOD acts as a matrix for enzyme immobilization. The redox PPF polymerized by a monomer of dimethlyaminomethlyferrocene (DAF) is directly deposited onto CNTs. The surface of the functionalized CNT has redox sites of ferrocene groups that shuttle electrons from CNTs to the sensing surface of the Au electrode. The synergy between the redox PPF and CNT provides benefits in terms of lowering the operational potential and enhancing the sensitivity (current). The optimized glucose biosensor revealed a sensitivity of 2.0μAmM-1 cm -2 at +0.4 V vs Ag/AgCl, a linear dynamic range of 4.9-27mM, and a response time of 5 s.
| 元の言語 | English |
|---|---|
| 記事番号 | 085202 |
| ジャーナル | Japanese Journal of Applied Physics |
| 巻 | 50 |
| 発行部数 | 8 PART 1 |
| DOI | |
| 出版物ステータス | Published - 2011 8 |
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ASJC Scopus subject areas
- Engineering(all)
- Physics and Astronomy(all)
これを引用
Amperometric biosensor based on carbon nanotube functionalized by redox plasma-polymerized film. / Hoshino, Tatsuya; Muguruma, Hitoshi.
：: Japanese Journal of Applied Physics, 巻 50, 番号 8 PART 1, 085202, 08.2011.研究成果: Article
}
TY - JOUR
T1 - Amperometric biosensor based on carbon nanotube functionalized by redox plasma-polymerized film
AU - Hoshino, Tatsuya
AU - Muguruma, Hitoshi
PY - 2011/8
Y1 - 2011/8
N2 - A novel fabrication approach for the amperometric biosensor based on multilayer films containing carbon nanotubes (CNT), a plasma-polymerized film (PPF), and enzyme glucose oxidase (GOD) is reported. The configuration of the electrochemical electrode is sequentially composed of sputtered gold, lower acetonitrile PPF, CNT, redox PPF, GOD, and upper acetonitrile PPF (denoted as PPF/GOD/Redox-PPF/CNT/PPF/Au). The lower acetonitrile PPF deposited on Au acts as a permselective membrane, and as a scaffold for CNT layer formation. The upper acetonirile PPF directly deposited on GOD acts as a matrix for enzyme immobilization. The redox PPF polymerized by a monomer of dimethlyaminomethlyferrocene (DAF) is directly deposited onto CNTs. The surface of the functionalized CNT has redox sites of ferrocene groups that shuttle electrons from CNTs to the sensing surface of the Au electrode. The synergy between the redox PPF and CNT provides benefits in terms of lowering the operational potential and enhancing the sensitivity (current). The optimized glucose biosensor revealed a sensitivity of 2.0μAmM-1 cm -2 at +0.4 V vs Ag/AgCl, a linear dynamic range of 4.9-27mM, and a response time of 5 s.
AB - A novel fabrication approach for the amperometric biosensor based on multilayer films containing carbon nanotubes (CNT), a plasma-polymerized film (PPF), and enzyme glucose oxidase (GOD) is reported. The configuration of the electrochemical electrode is sequentially composed of sputtered gold, lower acetonitrile PPF, CNT, redox PPF, GOD, and upper acetonitrile PPF (denoted as PPF/GOD/Redox-PPF/CNT/PPF/Au). The lower acetonitrile PPF deposited on Au acts as a permselective membrane, and as a scaffold for CNT layer formation. The upper acetonirile PPF directly deposited on GOD acts as a matrix for enzyme immobilization. The redox PPF polymerized by a monomer of dimethlyaminomethlyferrocene (DAF) is directly deposited onto CNTs. The surface of the functionalized CNT has redox sites of ferrocene groups that shuttle electrons from CNTs to the sensing surface of the Au electrode. The synergy between the redox PPF and CNT provides benefits in terms of lowering the operational potential and enhancing the sensitivity (current). The optimized glucose biosensor revealed a sensitivity of 2.0μAmM-1 cm -2 at +0.4 V vs Ag/AgCl, a linear dynamic range of 4.9-27mM, and a response time of 5 s.
UR - http://www.scopus.com/inward/record.url?scp=80052004703&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052004703&partnerID=8YFLogxK
U2 - 10.1143/JJAP.50.085202
DO - 10.1143/JJAP.50.085202
M3 - Article
AN - SCOPUS:80052004703
VL - 50
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
SN - 0021-4922
IS - 8 PART 1
M1 - 085202
ER -