Amperometric detection of polyphenols using peroxidase-immobilized gold electrodes

Shin Ichiro Imabayashi, Young Tae Kong, Masayoshi Watanabe

研究成果: Article

5 引用 (Scopus)

抜粋

Horseradish peroxidase (HRP) is covalently immobilized on a self-assembled monolayer of mercaptopropionic acid on vapor-deposited gold electrode. The electrode allows the polyphenol detection down to 2 μmol dm-3 with a linear relationship up to 25 μmol dm-3. The reduction current of oxidized polyphenol intermediate formed during the enzymatic reaction in the presence of H2O2 is proportional to the concentration of polyphenol. The present method is applicable to the determination of the total polyphenol content in wine and tea.

元の言語English
ページ(範囲)1020-1021
ページ数2
ジャーナルChemistry Letters
発行部数9
DOI
出版物ステータスPublished - 2000
外部発表Yes

ASJC Scopus subject areas

  • Chemistry(all)

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