Antibody immobilization on functional monolayers using a quartz crystal microbalance

Hidenobu Aizawa, Yasutoshi Gokita, Jong Won Park, Yasuo Yoshimi, Shigeru Kurosawa

研究成果: Article査読

7 被引用数 (Scopus)

抄録

This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.

本文言語English
論文番号1703460
ページ(範囲)1052-1056
ページ数5
ジャーナルIEEE Sensors Journal
6
5
DOI
出版ステータスPublished - 2006 10

ASJC Scopus subject areas

  • 器械工学
  • 電子工学および電気工学

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