Antibody immobilization on functional monolayers using a quartz crystal microbalance

Hidenobu Aizawa, Yasutoshi Gokita, Jong Won Park, Yasuo Yoshimi, Shigeru Kurosawa

研究成果: Article

7 引用 (Scopus)

抄録

This paper evaluated immobilization of anti-C-reactive protein (CRP) monoclonal antibody on a quartz crystal microbalance (QCM) when 2-aminoethanethiol (AET), 4,4'-dithiodibutyric acid (DDA), and 11-mercaptoundecanoic acid (MUA) were deposited on the gold surface of QCM. In all monolayers, anti-CRP antibodies were immobilized such as Langmuir types because it had been introduced with a corresponding active group. According to the Langmuir isotherm equation amax, the maximum immobilized amounts of anti-CRP antibody were 4.27, 2.72, and 3.74 pmol/cm2, respectively. Although the immobilized amount of anti-CRP antibody was highest on the AET monolayer, the amount of antigen-antibody binding between the anti-CRP antibody and the CRP was highest on the MUA monolayer. CRP was detected from negative to positive levels when the calibration curve was achieved using MUA monolayer.

元の言語English
記事番号1703460
ページ(範囲)1052-1056
ページ数5
ジャーナルIEEE Sensors Journal
6
発行部数5
DOI
出版物ステータスPublished - 2006 10

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Quartz crystal microbalances
quartz crystals
antibodies
immobilization
Antibodies
microbalances
Monolayers
proteins
Proteins
acids
Acids
Monoclonal antibodies
antigens
Antigens
Isotherms
isotherms
Gold
Calibration
gold
curves

ASJC Scopus subject areas

  • Engineering(all)
  • Electrical and Electronic Engineering

これを引用

Antibody immobilization on functional monolayers using a quartz crystal microbalance. / Aizawa, Hidenobu; Gokita, Yasutoshi; Park, Jong Won; Yoshimi, Yasuo; Kurosawa, Shigeru.

:: IEEE Sensors Journal, 巻 6, 番号 5, 1703460, 10.2006, p. 1052-1056.

研究成果: Article

Aizawa, Hidenobu ; Gokita, Yasutoshi ; Park, Jong Won ; Yoshimi, Yasuo ; Kurosawa, Shigeru. / Antibody immobilization on functional monolayers using a quartz crystal microbalance. :: IEEE Sensors Journal. 2006 ; 巻 6, 番号 5. pp. 1052-1056.
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