Bacterium-based heavy metal biosorbents: Enhanced uptake of cadmium by E. coli Expressing a metallothionein fused to β-galactosidase

Naoto Yoshida, T. Kato, T. Yoshida, K. Ogawa, Mitsuo Yamashita, Y. Murooka

研究成果: Article

14 引用 (Scopus)

抄録

We investigated the potential utility of a recombinant E. coli that expresses the human metallothionein II gene as a fusion protein with β-galactosidase as a heavy metal biosorbent. E. coli cells expressing the metallothionein fusion demonstrated enhanced binding of Cd2+ compared to cells that lack the metallothionein. It was shown that the metallothionein fusion was capable of efficiently removing Cd2+ from solutions. Approximately 40% of the Cd2+ accumulated by the recombinant cells free in suspension was associated with the outer cell membrane, and 60% of that was present in the cytoplasm.

元の言語English
ページ(範囲)551-558
ページ数8
ジャーナルBioTechniques
32
発行部数3
出版物ステータスPublished - 2002
外部発表Yes

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Galactosidases
Metallothionein
Heavy Metals
Cadmium
Escherichia coli
Bacteria
Fusion reactions
Cell membranes
Suspensions
Genes
Cells
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Bacterium-based heavy metal biosorbents : Enhanced uptake of cadmium by E. coli Expressing a metallothionein fused to β-galactosidase. / Yoshida, Naoto; Kato, T.; Yoshida, T.; Ogawa, K.; Yamashita, Mitsuo; Murooka, Y.

:: BioTechniques, 巻 32, 番号 3, 2002, p. 551-558.

研究成果: Article

Yoshida, Naoto ; Kato, T. ; Yoshida, T. ; Ogawa, K. ; Yamashita, Mitsuo ; Murooka, Y. / Bacterium-based heavy metal biosorbents : Enhanced uptake of cadmium by E. coli Expressing a metallothionein fused to β-galactosidase. :: BioTechniques. 2002 ; 巻 32, 番号 3. pp. 551-558.
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