Bio-fluorometric realtime gas-imaging system (sniff-cam) for skin ethanol vapor

Kenta Iitani, Toshiyuki Sato, Koji Toma, Takahiro Arakawa, Kohji Mitsubayashi

研究成果: Conference contribution

抄録

We developed gas-imaging system (sniff-cam) that allowed imaging of gaseous ethanol (EtOH). In the EtOH sniff-cam, a nicotinamide adenine dinucleotide (NAD)-dependent alcohol dehydrogenase (ADH) was used. When applying gaseous EtOH to an ADH-immobilized mesh with NAD+ solution, NADH was produced through an ADH-mediated reaction. NADH emits fluorescence at wavelength of 490 nm by UV excitation at 340 nm; thus concentration distribution of EtOH was visualized by measuring the distribution of fluorescence light intensity from NADH on the ADH-immobilized mesh surface. The dynamic range of EtOH sniff-cam was 0.1-1000 ppm. Besides, rounded fluorescence gradient centered in the gas outlet was observed. Results suggested that the EtOH sniff-cam could be applied to investigate the spatiotemporal change of EtOH emanated from the skin.

本文言語English
ホスト出版物のタイトルISOEN 2019 - 18th International Symposium on Olfaction and Electronic Nose, Proceedings
出版社Institute of Electrical and Electronics Engineers Inc.
ISBN(電子版)9781538683279
DOI
出版ステータスPublished - 2019 5月
外部発表はい
イベント18th International Symposium on Olfaction and Electronic Nose, ISOEN 2019 - Fukuoka, Japan
継続期間: 2019 5月 262019 5月 29

出版物シリーズ

名前ISOEN 2019 - 18th International Symposium on Olfaction and Electronic Nose, Proceedings

Conference

Conference18th International Symposium on Olfaction and Electronic Nose, ISOEN 2019
国/地域Japan
CityFukuoka
Period19/5/2619/5/29

ASJC Scopus subject areas

  • 信号処理
  • 器械工学

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