TY - GEN
T1 - Bio-fluorometric realtime gas-imaging system (sniff-cam) for skin ethanol vapor
AU - Iitani, Kenta
AU - Sato, Toshiyuki
AU - Toma, Koji
AU - Arakawa, Takahiro
AU - Mitsubayashi, Kohji
N1 - Funding Information:
This work was supported by the JSPS KAKENHI grant numbers JP17H01759, JP16J9604, the Japan Science and Technology Agency (JST) , and the Ministry of Education, Culture, Sports, Science and Technology (MEXT) Special Finds for “Cooperative Research Project of research center for Biomedical Engineering”
Publisher Copyright:
© 2019 IEEE.
PY - 2019/5
Y1 - 2019/5
N2 - We developed gas-imaging system (sniff-cam) that allowed imaging of gaseous ethanol (EtOH). In the EtOH sniff-cam, a nicotinamide adenine dinucleotide (NAD)-dependent alcohol dehydrogenase (ADH) was used. When applying gaseous EtOH to an ADH-immobilized mesh with NAD+ solution, NADH was produced through an ADH-mediated reaction. NADH emits fluorescence at wavelength of 490 nm by UV excitation at 340 nm; thus concentration distribution of EtOH was visualized by measuring the distribution of fluorescence light intensity from NADH on the ADH-immobilized mesh surface. The dynamic range of EtOH sniff-cam was 0.1-1000 ppm. Besides, rounded fluorescence gradient centered in the gas outlet was observed. Results suggested that the EtOH sniff-cam could be applied to investigate the spatiotemporal change of EtOH emanated from the skin.
AB - We developed gas-imaging system (sniff-cam) that allowed imaging of gaseous ethanol (EtOH). In the EtOH sniff-cam, a nicotinamide adenine dinucleotide (NAD)-dependent alcohol dehydrogenase (ADH) was used. When applying gaseous EtOH to an ADH-immobilized mesh with NAD+ solution, NADH was produced through an ADH-mediated reaction. NADH emits fluorescence at wavelength of 490 nm by UV excitation at 340 nm; thus concentration distribution of EtOH was visualized by measuring the distribution of fluorescence light intensity from NADH on the ADH-immobilized mesh surface. The dynamic range of EtOH sniff-cam was 0.1-1000 ppm. Besides, rounded fluorescence gradient centered in the gas outlet was observed. Results suggested that the EtOH sniff-cam could be applied to investigate the spatiotemporal change of EtOH emanated from the skin.
KW - Fluorescence
KW - UV-LED
KW - alcohol oxidase
KW - ethanol
KW - gas-imaging
UR - http://www.scopus.com/inward/record.url?scp=85072955144&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85072955144&partnerID=8YFLogxK
U2 - 10.1109/ISOEN.2019.8823482
DO - 10.1109/ISOEN.2019.8823482
M3 - Conference contribution
AN - SCOPUS:85072955144
T3 - ISOEN 2019 - 18th International Symposium on Olfaction and Electronic Nose, Proceedings
BT - ISOEN 2019 - 18th International Symposium on Olfaction and Electronic Nose, Proceedings
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 18th International Symposium on Olfaction and Electronic Nose, ISOEN 2019
Y2 - 26 May 2019 through 29 May 2019
ER -