Calcium measurement method by fluorescent analyses using a glass micro injector

S. Nagasawa, I. Shimoyama

研究成果: Conference contribution

2 被引用数 (Scopus)

抄録

We propose a calcium measurement using a glass micro injector. A fluorescent dye is injected into a small area through the injector. Since a light fiber probe is placed closely at the injector tip, fluorescence depending on calcium concentration is locally detected through the probe. This method is minimally invasive for living preparations because a calcium indicator is injected only for a short time. Also this measurement avoids the photo-bleaching effect by injecting fresh dye. The relationship between calcium concentration and fluorescence was confirmed in experiment. The dose of dye is controlled by the current applied to the injector. We also obtained the relationship between the amount of the injected dye and the current. Calcium can be measured only in several micrometers when the current is limited up to 7.67 nA. Finally, a prototype device has been fabricated using MEMS technology. A photo-diode and an electrode of the injector were built together in this device.

本文言語English
ホスト出版物のタイトルTRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
出版社Institute of Electrical and Electronics Engineers Inc.
ページ111-114
ページ数4
ISBN(電子版)0780377311, 9780780377318
DOI
出版ステータスPublished - 2003 1 1
外部発表はい
イベント12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers - Boston, United States
継続期間: 2003 6 82003 6 12

出版物シリーズ

名前TRANSDUCERS 2003 - 12th International Conference on Solid-State Sensors, Actuators and Microsystems, Digest of Technical Papers
1

Other

Other12th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS 2003 - Digest of Technical Papers
CountryUnited States
CityBoston
Period03/6/803/6/12

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

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