An amperometric enzyme biosensor fabricated with carbon nanotubes (CNTs) and plasma-polymerized thin films (PPFs) is reported. A mixture of the enzyme glucose oxidase (GOD) and a CNT film is sandwiched with 10-nm-thick acetonitrile PPFs. Under PPF layer was deposited onto a sputtered gold electrode. To facilitate the electrochemical communication between the CNT layer and GOD, CNT was treated with oxygen plasma. The device with single-walled CNTs showed a sensitivity higher than that of multi walled CNTs. The glucose biosensor showed ultrasensitivity (a sensitivity of 40μA mM-1 cm-2, a correlation coefficient of 0.992, a linear response range of 0.025 -1.9 mM, a detection limit of 6.2 μM at S/N = 3, +0.8 V vs Ag/AgCl), and a rapid response (<4 s in reaching 95% of maximum response). This high performance is attributed to the fact that CNTs have excellent electrocatalytic activity and enhance electron transfer, and that PPFs and/or the plasma process for CNTs are the enzyme-friendly platform, i.e., a suitable design of the interface between GOD and CNTs.
|ジャーナル||Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers|
|出版ステータス||Published - 2007 9月 7|
ASJC Scopus subject areas