Cerium(IV)-oligoDNA hybrid as highly selective artificial nuclease

Makoto Komiyama, Yota Takahashi, Naoya Takeda, Kazunari Matsumura, Teruyuki Kodama

研究成果: Article査読

39 被引用数 (Scopus)

抄録

Cerium(IV) ion is attached to the 5′ of a 19-mer DNA by use of iminodiacetate ligand. At pH 7.2 and 30 °C, the hybrid selectively cuts a 40-mer DNA, which has 19-mer sequence complementary with the DNA in the hybrid, at the target site (the 3′-side of the 19-mer portion of the substrate DNA). Cerium(IV) ion hydrolyzes thymidyly1(3′-5)thymidine to thymidine without concurrent oxidative cleavage of the ribose, indicating that the selective scission by the hybrid proceeds via the hydrolysis of the phosphodiester linkage.

本文言語English
ページ(範囲)31-34
ページ数4
ジャーナルSupramolecular Chemistry
4
1
DOI
出版ステータスPublished - 1994 1
外部発表はい

ASJC Scopus subject areas

  • Chemistry(all)

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