Characterization of immunoregulatory T cells and lymphocytophilic antibodies in ulcerative colitis: Analysis with monoclonal antibodies

S. Aiso, M. Watanabe, T. Hibi, T. Yoshida, M. Tsuchiya, S. Tsuru

研究成果: Article

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Using monoclonal antibodies to the surface antigens or suppressor/cytotoxic (Anti-Leu-2a) and helper/inducer (Anti-Leu-3a) T cell subsets, we characterized peripheral lymphocytes in 27 patients with ulcerative colitis (UC) and 15 age-matched healthy controls by a fluorescence activated cell sorter. The patients with active UC had a reduced percentage (8.2 ± 3.6%) of Anti-Leu-2a reactive subset in comparison with healthy controls (24.6 ± 3.6%) (P<0.01). No significant change was found in the percentage of Anti-Leu-3a reactive T cells between the patients and the controls. Moreover, the relationship of lymphocytophilic antibodies found in the patients with UC to the T cell subsets was also examined. After a fraction of normal T cells was eliminated with treatment of the sera from patients with active UC, the percentage of T cells reactive with Anti-Leu-2a decreased from 25 to 15%, whereas the percentage of T cells reactive with Anti-Leu-3a increased from 40 to 70%. In the reciprocal study, the lymphocytophilic antibodies reacted with 80% of Leu-3a negative T cells and 65% of Leu-2a negative T cells. These results demonstrated that the lymphocytophilic antibodies found in UC patients are reactive with suppressor T cells and thus play an important role in the loss of peripheral suppressor cells in the active UC patients. The lymphocytophilic antibodies were also demonstrated to be reactive with non-helper, non-suppressor T cell subsets and some populations of helper T cells. These studies suggest the presence of immune-regulatory disturbances contributing the pathogenesis of UC.

元の言語English
ページ(範囲)109-112
ページ数4
ジャーナルJournal of Clinical and Laboratory Immunology
9
発行部数2
出版物ステータスPublished - 1982 12 1

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ASJC Scopus subject areas

  • Immunology

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