Characterization of the nuclear large-subunit rRNA-encoding gene and the group-I self-splicing intron from Chlorella ellipsoidea C-87

Aimi Tadanori, Yamada Takashi, Yamashita Mitsuo, Murooka Yoshikatsu

研究成果: Article

6 引用 (Scopus)

抜粋

We found two group-I self-splicing introns in both the large subunit (LSU) and small subunit (SSU) of the nuclear rRNA-encoding genes (rDNA) of the unicellular green alga, Chlorella ellipsoidea C-87 (Ce). The primary and secondary structures of the LSU rRNA (3350 nt) and its intron (445 nt) were characterized. The intron was inserted in the conserved stem 32 of the LSU rRNA and contained all P1-P10 motifs of the group-IB intron. In vitro transcripts of the LSU rDNA containing the intron sequence displayed a strong self-splicing activity at high salt concentrations. The overall structure and splicing conditions of the LSU rRNA intron were, however, considerably different from those of the SSU rRNA intron of the same organism. These results suggest different origins and/or different evolutionary courses of these Ce introns.

元の言語English
ページ(範囲)139-144
ページ数6
ジャーナルGene
145
発行部数1
DOI
出版物ステータスPublished - 1994 7 22
外部発表Yes

ASJC Scopus subject areas

  • Genetics

フィンガープリント Characterization of the nuclear large-subunit rRNA-encoding gene and the group-I self-splicing intron from Chlorella ellipsoidea C-87' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用