Chlamydomonas CAV2 Encodes a Voltage- Dependent Calcium Channel Required for the Flagellar Waveform Conversion

Kenta Fujiu, Yoshitaka Nakayama, Ayaka Yanagisawa, Masahiro Sokabe, Kenjiro Yoshimura

研究成果: Article査読

66 被引用数 (Scopus)

抄録

Cilia and flagella can alter their beating patterns through changes in membrane excitation mediated by Ca2+ influx [1-6]. The ion channel that generates this Ca2+ influx and its cellular distribution have not been identified. In this study, we analyzed the Chlamydomonas ppr2 mutant, which is deficient in the production of a flagellar Ca2+ current and consequently has a defective photophobic response [7] and mechanoshock response. ppr2 had a mutation in CAV2, which encodes a homolog of the α1 subunit of voltage-dependent calcium channels (VDCCs). CAV2 has four domains, each with six transmembrane segments and EEEE loci in the ion-selective filter, which are typical of VDCCs in vertebrates. Interestingly, we found that CAV2 primarily localized toward the distal part of flagella. We provide evidence that CAV2 is transported toward the flagellar tip via intraflagellar transport (IFT) because CAV2 accumulated near the flagellar base when IFT was blocked. The results of this study suggest that the Ca2+ influx of Chlamydomonas flagella is mediated by the VDCC, CAV2, whose distribution is biased to the distal region of the flagellum.

本文言語English
ページ(範囲)133-139
ページ数7
ジャーナルCurrent Biology
19
2
DOI
出版ステータスPublished - 2009 1月 27
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)

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