Cloning and characterization of Xenopus laevis xSox7 cDNA

Masahide Shiozawa; Yoshiki Hiraoka; Naoki Komatsu; Motoyuki Ogawa; Yukinao Sakai; Sadakazu Aiso

doi:10.1016/S0167-4781(96)00145-5

Cloning and characterization of Xenopus laevis xSox7 cDNA

Masahide Shiozawa, Yoshiki Hiraoka, Naoki Komatsu, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

研究成果: Article › 査読

26 被引用数 (Scopus)

抄録

A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

本文言語	English
ページ（範囲）	73-76
ページ数	4
ジャーナル	Biochimica et Biophysica Acta - Gene Structure and Expression
巻	1309
号	1-2
DOI	https://doi.org/10.1016/S0167-4781(96)00145-5
出版ステータス	Published - 1996 11月 11
外部発表	はい

ASJC Scopus subject areas

構造生物学
生物理学
生化学
遺伝学

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10.1016/S0167-4781(96)00145-5

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title = "Cloning and characterization of Xenopus laevis xSox7 cDNA",

abstract = "A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.",

keywords = "(Xenopus laevis), Nucleotide sequence, SOX, SRY, cDNA",

author = "Masahide Shiozawa and Yoshiki Hiraoka and Naoki Komatsu and Motoyuki Ogawa and Yukinao Sakai and Sadakazu Aiso",

note = "Funding Information: We thank Dr. Chang Sim for reading the manuscript. This work was supported in part by a grant from the Ministry of Education, Science and Culture to S.A. and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research from Keio University to Y.H.",

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doi = "10.1016/S0167-4781(96)00145-5",

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AU - Shiozawa, Masahide

AU - Hiraoka, Yoshiki

AU - Komatsu, Naoki

AU - Ogawa, Motoyuki

AU - Sakai, Yukinao

AU - Aiso, Sadakazu

N1 - Funding Information: We thank Dr. Chang Sim for reading the manuscript. This work was supported in part by a grant from the Ministry of Education, Science and Culture to S.A. and by Keio Gakuji Fukuzawa Memorial Funds for the Advancement of Education and Research from Keio University to Y.H.

PY - 1996/11/11

Y1 - 1996/11/11

N2 - A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

AB - A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

KW - (Xenopus laevis)

KW - Nucleotide sequence

KW - SOX

KW - SRY

KW - cDNA

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Cloning and characterization of Xenopus laevis xSox7 cDNA

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