抄録
A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.
本文言語 | English |
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ページ(範囲) | 73-76 |
ページ数 | 4 |
ジャーナル | Biochimica et Biophysica Acta - Gene Structure and Expression |
巻 | 1309 |
号 | 1-2 |
DOI | |
出版ステータス | Published - 1996 11月 11 |
外部発表 | はい |
ASJC Scopus subject areas
- 構造生物学
- 生物理学
- 生化学
- 遺伝学