CarBaBb, the class III extradiol dioxygenase involved in carbazole degradation by Pseudomonas resinovorans CA10, was crystallized at 278 K by the hanging-drop vapour-diffusion method using PEG MME 550 as a precipitant. The crystals had a transparent thin square-pillar shape and belonged to space group P21212, with unit-cell parameters a = 122.8, b = 144.6, c = 49.2 Å, α = β = γ = 90°. The crystals diffracted to a maximum resolution of 1.9 Å and gave a data set with an overall R merge of 5.7% and a completeness of 98.6%. The VM value was 2.52 Å3 Da-1, which indicated a solvent content of 51.2%.
|ジャーナル||Acta Crystallographica Section D: Biological Crystallography|
|出版ステータス||Published - 2004 12 1|
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