D-Ser-containing humanin shows promotion of fibril formation

Kanehiro Hayashi, Jumpei Sasabe, Tomohiro Chiba, Sadakazu Aiso, Naoko Utsunomiya-Tate

研究成果: Article

抄録

Humanin (HN), a peptide of 24 amino acid residues, suppresses the neuronal cell death that is induced by the gene products of Alzheimer's disease. HN contains two Ser residues at positions 7 and 14. Because the proportion of d-Ser isomerized from l-Ser in proteins appears to increase as cellular organs age, we explored the structural effects of the isomerization of each Ser residue in HN. By using a thioflavin-T assay to detect fibril formation, we found that an HN derivative that contained two isomerized d-Ser residues had a greater tendency to form fibrils than did wild-type HN or HNs containing single d-Ser residues. A previous report showed that HN containing two d-Ser residues exerts neuroprotective activity. Our data, therefore, suggest that the fibril formation by HN that contains two d-Ser residues may promote HN neuroprotective activity.

元の言語English
ページ(範囲)2293-2297
ページ数5
ジャーナルAmino Acids
42
発行部数6
DOI
出版物ステータスPublished - 2012 6 1
外部発表Yes

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humanin
Cell death
Isomerization
Assays
Genes
Derivatives
Amino Acids
Peptides
Proteins
thioflavin T

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

Hayashi, K., Sasabe, J., Chiba, T., Aiso, S., & Utsunomiya-Tate, N. (2012). D-Ser-containing humanin shows promotion of fibril formation. Amino Acids, 42(6), 2293-2297. https://doi.org/10.1007/s00726-011-0971-6

D-Ser-containing humanin shows promotion of fibril formation. / Hayashi, Kanehiro; Sasabe, Jumpei; Chiba, Tomohiro; Aiso, Sadakazu; Utsunomiya-Tate, Naoko.

:: Amino Acids, 巻 42, 番号 6, 01.06.2012, p. 2293-2297.

研究成果: Article

Hayashi, K, Sasabe, J, Chiba, T, Aiso, S & Utsunomiya-Tate, N 2012, 'D-Ser-containing humanin shows promotion of fibril formation', Amino Acids, 巻. 42, 番号 6, pp. 2293-2297. https://doi.org/10.1007/s00726-011-0971-6
Hayashi, Kanehiro ; Sasabe, Jumpei ; Chiba, Tomohiro ; Aiso, Sadakazu ; Utsunomiya-Tate, Naoko. / D-Ser-containing humanin shows promotion of fibril formation. :: Amino Acids. 2012 ; 巻 42, 番号 6. pp. 2293-2297.
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