Determination of lactate by sequential injection analysis using a fluoride ion-selective electrode detector

Yuichi Takahashi, Takashi Masadome

研究成果: Article査読

抄録

A combination of sequential Injection Analysis (SIA) system and the ion-selective electrode (ISE) detector is considered to be one of a very effective analytical method. Reaction of lactate with lactate oxidase produces pyruvic acid and hydrogen peroxide. When the produced hydrogen peroxide is reacted with 4-aminoantipyrine (4-AAP) and 4-fluorophenol (4-FP) in the presence of horseradish peroxidase (HRP), F ion is produced. The concentration of lactate can be indirectly determined by measuring the F ion by F ISE. Based on this principle of detection of lactate, a new measurement method of lactate using an SIA with ISE detector was developed.

本文言語English
ページ(範囲)522-524
ページ数3
ジャーナルDenki Kagaku
88
6
DOI
出版ステータスPublished - 2020 11 5

ASJC Scopus subject areas

  • 電気化学

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