Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring

Maya Kamao, Yoshihisa Hirota, Yoshitomo Suhara, Naoko Tsugawa, Kimie Nakagawa, Toshio Okano, Hiroshi Hasegawa

研究成果: Comment/debate

4 引用 (Scopus)

抄録

This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

元の言語English
ページ(範囲)863-867
ページ数5
ジャーナルAnalytical Sciences
33
発行部数7
DOI
出版物ステータスPublished - 2017

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Vitamin K 3
Liquid chromatography
Mass spectrometry
Monitoring
Assays
Ions
Deuterium
Culture Media
Plasmas
Recovery

ASJC Scopus subject areas

  • Analytical Chemistry

これを引用

Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring. / Kamao, Maya; Hirota, Yoshihisa; Suhara, Yoshitomo; Tsugawa, Naoko; Nakagawa, Kimie; Okano, Toshio; Hasegawa, Hiroshi.

:: Analytical Sciences, 巻 33, 番号 7, 2017, p. 863-867.

研究成果: Comment/debate

Kamao, Maya ; Hirota, Yoshihisa ; Suhara, Yoshitomo ; Tsugawa, Naoko ; Nakagawa, Kimie ; Okano, Toshio ; Hasegawa, Hiroshi. / Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring. :: Analytical Sciences. 2017 ; 巻 33, 番号 7. pp. 863-867.
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abstract = "This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2{\%}, with the corresponding recoveries equaling 90.5 - 109.6{\%}. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.",
keywords = "LC-MS/MS, Menadione, Pseudo MRM, Vitamin K",
author = "Maya Kamao and Yoshihisa Hirota and Yoshitomo Suhara and Naoko Tsugawa and Kimie Nakagawa and Toshio Okano and Hiroshi Hasegawa",
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T1 - Determination of menadione by liquid chromatography-tandem mass spectrometry using pseudo multiple reaction monitoring

AU - Kamao, Maya

AU - Hirota, Yoshihisa

AU - Suhara, Yoshitomo

AU - Tsugawa, Naoko

AU - Nakagawa, Kimie

AU - Okano, Toshio

AU - Hasegawa, Hiroshi

PY - 2017

Y1 - 2017

N2 - This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

AB - This study aimed to develop a menadione (MD) determination method employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a pseudo multiple reaction monitoring (MRM) technique, wherein two quadrupoles are used to monitor the same ion. Detection limits of 40 and 2 pg were obtained for MD and its deuterium-labeled form, respectively, whereas MD intra- and inter-assay coefficient of variation values were determined as 5.4 - 8.2%, with the corresponding recoveries equaling 90.5 - 109.6%. The developed method enables determination of MD in urine, plasma, cell extract, and culture media, demonstrating that pseudo multiple reaction monitoring can achieve quantification of compounds forming no suitable product ions, such as MD.

KW - LC-MS/MS

KW - Menadione

KW - Pseudo MRM

KW - Vitamin K

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