Determination of plasma Vitamin K by high-performance liquid chromatography with fluorescence detection using Vitamin K analogs as internal standards

Maya Kamao, Yoshitomo Suhara, Naoko Tsugawa, Toshio Okano

研究成果: Article査読

58 被引用数 (Scopus)

抄録

A HPLC fluorescence determination method for Vitamin K derivatives (Vitamin K1, phylloquinone, PK and K2, menaquinones, MK-4 and MK-7) using post-column reduction and internal standards was developed. Selectivity and reproducibility were increased by optimized chromatography conditions and satisfactory precision and accuracy were attained by using synthetic internal standards. After addition of internal standards to plasma samples, lipids were extracted with ethanol and hexane. Chromatography was performed by isocratic reverse phase separation on a C18 column. Vitamin K derivatives were detected at 430 nm with excitation at 320 nm for MK-4 and 240 nm for PK and MK-7. The detection limits for MK-4, PK and MK-7 were 4, 2 and 4 pg, respectively. The recoveries of MK-4, PK and MK-7 were greater than 92% and the inter- and intra-assay R.S.D. values were 5.7-9.2% for MK-4, 4.9-9.6% for PK and 6.3-19.3% for MK-7. The data showed good correlation between proposed method and LC-APCI/MS method for MK-4 (R2 = 0.988), PK (R2 = 0.979) and MK-7 (R2 = 0.986). The method allows the determination of Vitamin K for evaluating their clinical and nutritional status.

本文言語English
ページ(範囲)41-48
ページ数8
ジャーナルJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
816
1-2
DOI
出版ステータスPublished - 2005 2月 25
外部発表はい

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 臨床生化学
  • 細胞生物学

フィンガープリント

「Determination of plasma Vitamin K by high-performance liquid chromatography with fluorescence detection using Vitamin K analogs as internal standards」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル