Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation

Yuichi Tada, Kimio Sumaru, Mitsuyoshi Kameda, Katsuhide Ohi, Toshiyuki Takagi, Toshiyuki Kanamori, Yasuo Yoshimi

研究成果: Article

24 引用 (Scopus)

抄録

Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350-400 nm, 35 mW/cm2, 5 min) even on the region where living cells exist. Cell adhesion was evaluated by observing BALB/3T3 fibroblasts, which had been seeded on PRCS and then irradiated with UV light regionally, after 12 h incubation and subsequent washing to remove scarcely adhering cells. As a result, it was confirmed that the number of remaining cells in irradiated region was at most 2.5 times greater than that in nonirradiated regions, suggesting the implementation of a novel scheme to manipulate living cells individually by light irradiation in a parallel and simultaneous manner. Influence of the composition of the polymer material consisting the substrate surface was also investigated systematically.

元の言語English
ページ(範囲)495-499
ページ数5
ジャーナルJournal of Applied Polymer Science
100
発行部数1
DOI
出版物ステータスPublished - 2006 4 5

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Cell adhesion
Cell culture
Irradiation
Ultraviolet radiation
Cells
Functional polymers
Fibroblasts
Washing
Polyethylene glycols
Polymers
Substrates
Chemical analysis

ASJC Scopus subject areas

  • Polymers and Plastics

これを引用

Development of a photoresponsive cell culture surface : Regional enhancement of living-cell adhesion induced by local light irradiation. / Tada, Yuichi; Sumaru, Kimio; Kameda, Mitsuyoshi; Ohi, Katsuhide; Takagi, Toshiyuki; Kanamori, Toshiyuki; Yoshimi, Yasuo.

:: Journal of Applied Polymer Science, 巻 100, 番号 1, 05.04.2006, p. 495-499.

研究成果: Article

Tada, Yuichi ; Sumaru, Kimio ; Kameda, Mitsuyoshi ; Ohi, Katsuhide ; Takagi, Toshiyuki ; Kanamori, Toshiyuki ; Yoshimi, Yasuo. / Development of a photoresponsive cell culture surface : Regional enhancement of living-cell adhesion induced by local light irradiation. :: Journal of Applied Polymer Science. 2006 ; 巻 100, 番号 1. pp. 495-499.
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AU - Ohi, Katsuhide

AU - Takagi, Toshiyuki

AU - Kanamori, Toshiyuki

AU - Yoshimi, Yasuo

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