Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation

Yuichi Tada, Kimio Sumaru, Mitsuyoshi Kameda, Katsuhide Ohi, Toshiyuki Takagi, Toshiyuki Kanamori, Yasuo Yoshimi

研究成果: Article査読

24 被引用数 (Scopus)

抄録

Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350-400 nm, 35 mW/cm2, 5 min) even on the region where living cells exist. Cell adhesion was evaluated by observing BALB/3T3 fibroblasts, which had been seeded on PRCS and then irradiated with UV light regionally, after 12 h incubation and subsequent washing to remove scarcely adhering cells. As a result, it was confirmed that the number of remaining cells in irradiated region was at most 2.5 times greater than that in nonirradiated regions, suggesting the implementation of a novel scheme to manipulate living cells individually by light irradiation in a parallel and simultaneous manner. Influence of the composition of the polymer material consisting the substrate surface was also investigated systematically.

本文言語English
ページ(範囲)495-499
ページ数5
ジャーナルJournal of Applied Polymer Science
100
1
DOI
出版ステータスPublished - 2006 4 5

ASJC Scopus subject areas

  • 化学 (全般)
  • 表面、皮膜および薄膜
  • ポリマーおよびプラスチック
  • 材料化学

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