Distribution of gamma-glutamyl transpeptidase in human pancreas: Immunohistochemical study with a monoclonal antibody

K. Yasuda, M. Shiozawa, Sadakazu Aiso, S. Taniguchi, S. Yamashita

研究成果: Article

10 引用 (Scopus)

抄録

We studied in detail the distribution pattern of γ-glutamyl transpeptidase (γ-GT) in human pancreas, using the immunoperoxidase technique and a monoclonal antibody to human kidney γ-GT. Positive reaction was confined exclusively to the luminal surface of the centroacinar cells and the epithelia of the intercalated, intralobular, and interlobular ducts. The immunoreaction was stronger in the intercalated and intralobular ducts than in the interlobular ducts. The acinar cells did not show any reaction. The islets of Langerhans were heavily surrounded by the ducts, and normal islet cells showed no reaction. The course of the ducts, from the acinar lumina to the interlobular ducts, was delineated by using reaction sites positive for γ-GT as markers. The courses of the ducts, which comprise the pathway for pancreatic juice, were found to vary widely in their connections with each other, especially between the intralobular and interlobular ducts. At least three separate routes could be identified.

元の言語English
ページ(範囲)339-350
ページ数12
ジャーナルJournal of Histochemistry and Cytochemistry
38
発行部数3
DOI
出版物ステータスPublished - 1990 1 1
外部発表Yes

ASJC Scopus subject areas

  • Anatomy
  • Histology

これを引用

Distribution of gamma-glutamyl transpeptidase in human pancreas : Immunohistochemical study with a monoclonal antibody. / Yasuda, K.; Shiozawa, M.; Aiso, Sadakazu; Taniguchi, S.; Yamashita, S.

:: Journal of Histochemistry and Cytochemistry, 巻 38, 番号 3, 01.01.1990, p. 339-350.

研究成果: Article

@article{2c7731c940464133a99237756ca26386,
title = "Distribution of gamma-glutamyl transpeptidase in human pancreas: Immunohistochemical study with a monoclonal antibody",
abstract = "We studied in detail the distribution pattern of γ-glutamyl transpeptidase (γ-GT) in human pancreas, using the immunoperoxidase technique and a monoclonal antibody to human kidney γ-GT. Positive reaction was confined exclusively to the luminal surface of the centroacinar cells and the epithelia of the intercalated, intralobular, and interlobular ducts. The immunoreaction was stronger in the intercalated and intralobular ducts than in the interlobular ducts. The acinar cells did not show any reaction. The islets of Langerhans were heavily surrounded by the ducts, and normal islet cells showed no reaction. The course of the ducts, from the acinar lumina to the interlobular ducts, was delineated by using reaction sites positive for γ-GT as markers. The courses of the ducts, which comprise the pathway for pancreatic juice, were found to vary widely in their connections with each other, especially between the intralobular and interlobular ducts. At least three separate routes could be identified.",
keywords = "Duct system, Human pancreas, Immunohistochemistry, Monoclonal antibody, γ-Glutamyl transpeptidase",
author = "K. Yasuda and M. Shiozawa and Sadakazu Aiso and S. Taniguchi and S. Yamashita",
year = "1990",
month = "1",
day = "1",
doi = "10.1177/38.3.1689336",
language = "English",
volume = "38",
pages = "339--350",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "3",

}

TY - JOUR

T1 - Distribution of gamma-glutamyl transpeptidase in human pancreas

T2 - Immunohistochemical study with a monoclonal antibody

AU - Yasuda, K.

AU - Shiozawa, M.

AU - Aiso, Sadakazu

AU - Taniguchi, S.

AU - Yamashita, S.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - We studied in detail the distribution pattern of γ-glutamyl transpeptidase (γ-GT) in human pancreas, using the immunoperoxidase technique and a monoclonal antibody to human kidney γ-GT. Positive reaction was confined exclusively to the luminal surface of the centroacinar cells and the epithelia of the intercalated, intralobular, and interlobular ducts. The immunoreaction was stronger in the intercalated and intralobular ducts than in the interlobular ducts. The acinar cells did not show any reaction. The islets of Langerhans were heavily surrounded by the ducts, and normal islet cells showed no reaction. The course of the ducts, from the acinar lumina to the interlobular ducts, was delineated by using reaction sites positive for γ-GT as markers. The courses of the ducts, which comprise the pathway for pancreatic juice, were found to vary widely in their connections with each other, especially between the intralobular and interlobular ducts. At least three separate routes could be identified.

AB - We studied in detail the distribution pattern of γ-glutamyl transpeptidase (γ-GT) in human pancreas, using the immunoperoxidase technique and a monoclonal antibody to human kidney γ-GT. Positive reaction was confined exclusively to the luminal surface of the centroacinar cells and the epithelia of the intercalated, intralobular, and interlobular ducts. The immunoreaction was stronger in the intercalated and intralobular ducts than in the interlobular ducts. The acinar cells did not show any reaction. The islets of Langerhans were heavily surrounded by the ducts, and normal islet cells showed no reaction. The course of the ducts, from the acinar lumina to the interlobular ducts, was delineated by using reaction sites positive for γ-GT as markers. The courses of the ducts, which comprise the pathway for pancreatic juice, were found to vary widely in their connections with each other, especially between the intralobular and interlobular ducts. At least three separate routes could be identified.

KW - Duct system

KW - Human pancreas

KW - Immunohistochemistry

KW - Monoclonal antibody

KW - γ-Glutamyl transpeptidase

UR - http://www.scopus.com/inward/record.url?scp=0025318874&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025318874&partnerID=8YFLogxK

U2 - 10.1177/38.3.1689336

DO - 10.1177/38.3.1689336

M3 - Article

C2 - 1689336

AN - SCOPUS:0025318874

VL - 38

SP - 339

EP - 350

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 3

ER -