TY - JOUR
T1 - Dorsal bipedicled island skin flap
T2 - A new flap model in mice
AU - Minh, Trinh Cao
AU - Ichioka, Shigeru
AU - Harii, Kiyonori
AU - Shibata, Masahiro
AU - Ando, Joji
AU - Nakatsuka, Takashi
PY - 2002
Y1 - 2002
N2 - Mice are popular animals for biomedical studies, but few skin flap models have been reported in them. To investigate the ischaemia/reperfusion phenomenon in skin flaps, we first investigated the vascular anatomy of murine dorsal skin and then designed a suitable murine dorsal skin flap model. In 120 mice, six distinct vascular patterns were identified, one being seen in 111 mice (93%). Based on this finding, in Part 2 of the study, 15 mice had flaps (4 x 4 cm) raised based on the two caudal vascular pedicles of the left and right deep circumflex iliac vessels as a bipedicled flap in which the mean (SD) survival was 96 (5)%. In a further 10 mice, flaps were raised based on a single pedicle, the left deep circumflex iliac vessel, as a monopedicled flap, in which the mean (SD) survival was 71 (12)%. The bipedicled flap model was then used to study ischaemia/reperfusion injury. Twenty flaps were subjected to eight hours of ischaemia and subsequent reperfusion, and their mean (SD) survival was 43 (26)%. Histological assessments were also carried out using neutrophil and leucocyte counts, and significant differences between groups were observed.
AB - Mice are popular animals for biomedical studies, but few skin flap models have been reported in them. To investigate the ischaemia/reperfusion phenomenon in skin flaps, we first investigated the vascular anatomy of murine dorsal skin and then designed a suitable murine dorsal skin flap model. In 120 mice, six distinct vascular patterns were identified, one being seen in 111 mice (93%). Based on this finding, in Part 2 of the study, 15 mice had flaps (4 x 4 cm) raised based on the two caudal vascular pedicles of the left and right deep circumflex iliac vessels as a bipedicled flap in which the mean (SD) survival was 96 (5)%. In a further 10 mice, flaps were raised based on a single pedicle, the left deep circumflex iliac vessel, as a monopedicled flap, in which the mean (SD) survival was 71 (12)%. The bipedicled flap model was then used to study ischaemia/reperfusion injury. Twenty flaps were subjected to eight hours of ischaemia and subsequent reperfusion, and their mean (SD) survival was 43 (26)%. Histological assessments were also carried out using neutrophil and leucocyte counts, and significant differences between groups were observed.
KW - Ischaemia/reperfusion injury
KW - Mouse
KW - Skin flap
UR - http://www.scopus.com/inward/record.url?scp=0036436164&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036436164&partnerID=8YFLogxK
U2 - 10.1080/028443102320791798
DO - 10.1080/028443102320791798
M3 - Article
C2 - 12477083
AN - SCOPUS:0036436164
VL - 36
SP - 262
EP - 267
JO - Scandinavian Journal of Plastic and Reconstructive Surgery
JF - Scandinavian Journal of Plastic and Reconstructive Surgery
SN - 2000-656X
IS - 5
ER -