Dual-target gas-phase biosensor (bio-sniffer) for assessment of lipid metabolism from breath acetone and isopropanol

Koji Toma, Masato Tsujii, Takahiro Arakawa, Yasuhiko Iwasaki, Kohji Mitsubayashi

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Volatile organic compounds (VOCs) in breath and skin gas are promising samples for non-invasive and simple disease screening and metabolism assessment. In addition, simultaneous measurement of multiple VOCs helps to improve the examination quality and allows for more reliable disease screening and investigation of detailed metabolic pathways. In this study, we have developed a dual-target gas-phase biosensor (bio-sniffer) that allows for measurement of isopropanol (IPA) and acetone vapours, relevant VOCs for lipid metabolisms, by simply exchanging coenzyme solutions. The measurement exploited a reversible redox reaction that was catalysed by secondary alcohol dehydrogenase (S-ADH). IPA/acetone was oxidised/reduced together with reduction/oxidation of a coenzyme, oxidised (NAD+)/reduced (NADH) form of β-nicotinamide adenine dinucleotide, depending on surrounding pH (8.5/7.5). This reaction resulted in producing/consuming NADH which exhibited autofluorescence (λex = 340 nm, λfl = 490 nm), by which IPA/acetone was measured. The characterization of the dual-target bio-sniffer showed the dynamic ranges for IPA and acetone vapour were 3.3–1000 ppb and 13.0–3000 ppb, respectively, which encompasses those in the breath of healthy people (IPA, 10–30 ppb; acetone, 200–900 ppb). Finally, the dual-target bio-sniffer was applied for measurement of IPA and acetone in the breath of healthy people. As with the standard IPA and acetone vapour, intermittent and repeated measurement of both VOCs in the breath was demonstrated. These results indicated that the dual-target bio-sniffer would be a useful tool to assess the lipid metabolism in detail by measuring temporal changes of IPA and acetone concentrations in the breath.

本文言語English
論文番号129260
ジャーナルSensors and Actuators, B: Chemical
329
DOI
出版ステータスPublished - 2021 2月 15
外部発表はい

ASJC Scopus subject areas

  • 電子材料、光学材料、および磁性材料
  • 器械工学
  • 凝縮系物理学
  • 表面、皮膜および薄膜
  • 金属および合金
  • 電子工学および電気工学
  • 材料化学

フィンガープリント

「Dual-target gas-phase biosensor (bio-sniffer) for assessment of lipid metabolism from breath acetone and isopropanol」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル