Effects of expression of hemA and hemB genes on production of porphyrin in Propionibacterium freudenreichii

Yongzhe Piao, Pornpimon Kiatpapan, Mitsuo Yamashita, Yoshikatsu Murooka

研究成果: Article

20 引用 (Scopus)

抜粋

The genus Propionibacterium has a wide range of probiotic activities that are exploited in dairy and fermentation systems such as cheeses, propionic acid, and tetrapyrrole compounds. In order to improve production of tetrapyrrole compounds, we expressed the hemA gene, which encodes δ-aminolevulinic acid (ALA) synthase from Rhodobacter sphaeroides, and the hemB gene, which encodes porphobilinogen (PBG) synthase from Propionibacterium freudenreichii subsp. shermanii IFO12424, either monocistronically or polycistronically in strain IFO12426. The recombinant strains accumulated larger amounts of ALA and PBG, with resultant 28- to 33-fold-higher production of porphyrinogens, such as uroporphyrinogen and coproporphyrinogen, than those observed in strain IFO12426, which harbored the shuttle vector pPK705.

元の言語English
ページ(範囲)7561-7566
ページ数6
ジャーナルApplied and Environmental Microbiology
70
発行部数12
DOI
出版物ステータスPublished - 2004 12

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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