TY - JOUR
T1 - Effects of shear stress on wound-healing angiogenesis in the rabbit ear chamber
AU - Ichioka, Shigeru
AU - Shibata, Masahiro
AU - Kosaki, Keisuke
AU - Sato, Yuko
AU - Harii, Kiyonori
AU - Kamiya, Akira
PY - 1997/9
Y1 - 1997/9
N2 - Recent studies indicate that wall shear stress plays a significant role in the physiological adaptation of the vascular system. This study focused on the effect of sustained wall shear stress on wound-healing angiogenesis by exploring the morphologic and hemodynamic changes in developing microvessels in vive through the tissue repair process. Rabbits were treated with the α1 blocker prazosin (50 mg/L in water) orally from Day 0 to Day 23 after implantation of ear chambers to increase peripheral blood flow. The microvasculature in the chamber was recorded from Day 7 to Day 23 by using an intravital videomicroscope. The relative area of the chamber covered by vascularized tissue (%), the rate of ingrowth (mm2/day), the total vascular area (mm2), and the wall shear stress level (dyne/cm2) in venules (diameter in 20-40 μm) were quantified using a computerized image analysis system. The relative area increased significantly in the prazosin-treated animals from Days 7 to 19. The chamber of the treated group was completely covered with vascularized tissue earlier than that of the control group. The final total vascular area was larger by 21% in the treated group. The time course of shear stress in the treated group showed an initial elevation (1.44 times increase vs the control) followed by a gradual decrease toward the control level. These findings suggest that Wound-healing angiogenesis may be partly involved in the adaptive response of microvasculature to shear stress.
AB - Recent studies indicate that wall shear stress plays a significant role in the physiological adaptation of the vascular system. This study focused on the effect of sustained wall shear stress on wound-healing angiogenesis by exploring the morphologic and hemodynamic changes in developing microvessels in vive through the tissue repair process. Rabbits were treated with the α1 blocker prazosin (50 mg/L in water) orally from Day 0 to Day 23 after implantation of ear chambers to increase peripheral blood flow. The microvasculature in the chamber was recorded from Day 7 to Day 23 by using an intravital videomicroscope. The relative area of the chamber covered by vascularized tissue (%), the rate of ingrowth (mm2/day), the total vascular area (mm2), and the wall shear stress level (dyne/cm2) in venules (diameter in 20-40 μm) were quantified using a computerized image analysis system. The relative area increased significantly in the prazosin-treated animals from Days 7 to 19. The chamber of the treated group was completely covered with vascularized tissue earlier than that of the control group. The final total vascular area was larger by 21% in the treated group. The time course of shear stress in the treated group showed an initial elevation (1.44 times increase vs the control) followed by a gradual decrease toward the control level. These findings suggest that Wound-healing angiogenesis may be partly involved in the adaptive response of microvasculature to shear stress.
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U2 - 10.1006/jsre.1997.5170
DO - 10.1006/jsre.1997.5170
M3 - Article
C2 - 9344711
AN - SCOPUS:0031238685
SN - 0022-4804
VL - 72
SP - 29
EP - 35
JO - Journal of Surgical Research
JF - Journal of Surgical Research
IS - 1
ER -