Electron transfer reaction of glucose oxidase hybrids modified with phenothiazine via poly(ethylene oxide) spacer on acidic amino acid residues

Sayuri Aoki, Kunikazu Ishii, Takeshi Ueki, Kazumichi Ban, Shin ichiro Imabayashi, Masayoshi Watanabe

研究成果: Article査読

9 被引用数 (Scopus)

抄録

GOx hybrids [GOx-(PT-PEO-NH2)] are prepared by covalently bonding phenothiazine(PT)-labeled poly(ethylene oxide) (PEO) oligomers having an amino end group, PT-PEO-NH2, to acidic amino acid residues on the enzyme surface. The rate constant for the mediated FADH/FADH2 oxidation calculated from the catalytic current under substrate-saturated conditions ranges from 1.7 to 388 s-1, and the largest value is obtained for GOx hybrids with PT-PEO of molecular weight 3000. Surprisingly effective electron transfer from FADH/FADH2 to PT+ is achieved in the GOx-(PT-PEO-NH2) hybrids due to the PT modification to aspartic or glutamic acid residues, many of which are located close to the FAD center.

本文言語English
ページ(範囲)256-257
ページ数2
ジャーナルChemistry Letters
2
DOI
出版ステータスPublished - 2002 2月 5
外部発表はい

ASJC Scopus subject areas

  • 化学 (全般)

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