Enhanced degradation of chitosan by applying plasma treatment in combination with oxidizing agents for potential use as an anticancer agent

Solution plasma (SP) treatment in combination with oxidizing agents, i.e., hydrogen peroxide (H₂O₂), potassium persulfate (K₂S₂O₈) and sodium nitrite (NaNO₂) were adopted to chitosan degradation in order to achieve fast degradation rate, low chemicals used and high yield of low-molecular-weight chitosan and chitooligosaccharide (COS). Among the studied oxidizing agents, H₂O₂ was found to be the best choice in terms of appreciable molecular weight reduction without major change in chemical structure of the degraded products of chitosan. By the combination with SP treatment, dilute solution of H₂O₂ (4–60 mM) was required for effective degradation of chitosan. The combination of SP treatment and dilute solution of H₂O₂ (60 mM) resulted in the great reduction of molecular weight of chitosan and water-soluble chitosan was obtained as a major product. The resulting water-soluble chitosan was precipitated to obtain COS. An inhibitory effect against cervical cancer cell line (HeLa cells) of COS was also examined.