TY - JOUR
T1 - Enormously fast RNA hydrolysis by Lanthanide(III) ions under physiological conditions
T2 - Eminent candidates for novel tools of biotechnology
AU - Matsumura, Kazunari
AU - Komiyama, Makoto
PY - 1997/8
Y1 - 1997/8
N2 - Lanthanide(III) ions have shown enormous catalyses for the hydrolysis of the phosphodiester linkages in RNA, indicating their high potential for versatile applications to biotechnology and molecular biology. The activity monotonically increases with increasing atomic number in the lanthanide series, the last three ions (Tm3+, Yb3+, and Lu3+) being the most active. Non-lanthanide metal ions are virtually inactive. The pseudo first-order rate constant for the hydrolysis of adenylyl (3'-5') adenosine (ApA)) by LuCl3, (5 mmol·dm-3) at pH 7.2 and 30°C is 1.9 x 10-1 min-1 (the half-life is only 3.6 min), corresponding to 108-fold acceleration. The product is an equimolar mixture of adenosine and its 2'- or 3'-monophosphate without any byproducts. The 2',3'-cyclic monophosphate of adenosine is not accumulated much in the reaction mixture. Lanthanide ions also efficiently hydrolyze oligoribonucleotides without a specific base-preference. In ApA hydrolysis by NdCl3, and GdCl3, the dependence of the hydrolysis rate on either the pH or concentration of the metal salt coincides fairly well with the corresponding profile of the equilibrium concentration of the bimetallic hydroxo-cluster [M2(OH)2]4+ (M = metal ion). Both the formation of the pentacoordinated intermediate and its decomposition are greatly promoted by lanthanide ions. A catalytic mechanism in which two metal ions (or their coordination water) in these tetracationic hydroxo-clusters show acid/base cooperation is proposed.
AB - Lanthanide(III) ions have shown enormous catalyses for the hydrolysis of the phosphodiester linkages in RNA, indicating their high potential for versatile applications to biotechnology and molecular biology. The activity monotonically increases with increasing atomic number in the lanthanide series, the last three ions (Tm3+, Yb3+, and Lu3+) being the most active. Non-lanthanide metal ions are virtually inactive. The pseudo first-order rate constant for the hydrolysis of adenylyl (3'-5') adenosine (ApA)) by LuCl3, (5 mmol·dm-3) at pH 7.2 and 30°C is 1.9 x 10-1 min-1 (the half-life is only 3.6 min), corresponding to 108-fold acceleration. The product is an equimolar mixture of adenosine and its 2'- or 3'-monophosphate without any byproducts. The 2',3'-cyclic monophosphate of adenosine is not accumulated much in the reaction mixture. Lanthanide ions also efficiently hydrolyze oligoribonucleotides without a specific base-preference. In ApA hydrolysis by NdCl3, and GdCl3, the dependence of the hydrolysis rate on either the pH or concentration of the metal salt coincides fairly well with the corresponding profile of the equilibrium concentration of the bimetallic hydroxo-cluster [M2(OH)2]4+ (M = metal ion). Both the formation of the pentacoordinated intermediate and its decomposition are greatly promoted by lanthanide ions. A catalytic mechanism in which two metal ions (or their coordination water) in these tetracationic hydroxo-clusters show acid/base cooperation is proposed.
KW - Lanthanide ion
KW - Lutetium(III) ion
KW - Metal hydroxo-cluster
KW - RNA hydrolysis
KW - Ytterbium(III) ion
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U2 - 10.1093/oxfordjournals.jbchem.a021765
DO - 10.1093/oxfordjournals.jbchem.a021765
M3 - Article
C2 - 9378718
AN - SCOPUS:0030799197
VL - 122
SP - 387
EP - 394
JO - Journal of Biochemistry
JF - Journal of Biochemistry
SN - 0021-924X
IS - 2
ER -