Enterolactone restricts the proliferation of the LNCaP human prostate cancer cell line in vitro

Mark J. McCann, Chris I.R. Gill, Trevor Linton, D. Berrar, Hugh McGlynn, Ian R. Rowland

研究成果: Article査読

25 被引用数 (Scopus)

抄録

Ecological data suggest a long-term diet high in plant material rich in biologically active compounds, such as the lignans, can significantly influence the development of prostate cancer over the lifetime of an individual. The capacity of a pure mammalian lignan, enterolactone (ENL), to influence the proliferation of the LNCaP human prostate cancer cell line was investigated as a function of cell density, metabolic activity, expression and secretion of prostate specific antigen (PSA), cell cycle profile, and the expression of genes involved in development and progression of prostate cancer. Treatment with a subcytotoxic concentration of ENL (60 μM for 72 h) was found to reduce: cell density (57.5%, SD 7.23, p < 0.001), metabolic activity (55%, SD 0.03, p < 0.001), secretion of PSA (48.50% SD 4.74, p = 0.05) and induce apoptosis (8.33-fold SD 0.04, p = 0.001) compared to untreated cells. Cotreatment with 10 μM etoposide was found to increase apoptosis by 50.17% (SD 0.02, p < 0.001). Additionally, several key genes (e. g. MCMs, survivin and CDKs) were beneficially regulated by ENL treatment (p < 0.05). The data suggest that the antiproliferative activity of ENL is a consequence of altered expression of cell cycle associated genes and provides novel molecular evidence for the antiproliferative properties of a pure lignan in prostate cancer.

本文言語English
ページ(範囲)567-580
ページ数14
ジャーナルMolecular Nutrition and Food Research
52
5
DOI
出版ステータスPublished - 2008 5月
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 食品科学

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