Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults

Hirohisa Tajima, Takako Niikura, Yuichi Hashimoto, Yuko Ito, Yoshiko Kita, Kenzo Terashita, Kazuto Yamazaki, Atsuo Koto, Sadakazu Aiso, Ikuo Nishimoto

研究成果: Article査読

109 被引用数 (Scopus)

抄録

An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.

本文言語English
ページ(範囲)227-231
ページ数5
ジャーナルNeuroscience Letters
324
3
DOI
出版ステータスPublished - 2002 5 24
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)

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