Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults

Hirohisa Tajima, Takako Niikura, Yuichi Hashimoto, Yuko Ito, Yoshiko Kita, Kenzo Terashita, Kazuto Yamazaki, Atsuo Koto, Sadakazu Aiso, Ikuo Nishimoto

研究成果: Article

102 引用 (Scopus)

抄録

An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.

元の言語English
ページ(範囲)227-231
ページ数5
ジャーナルNeuroscience Letters
324
発行部数3
DOI
出版物ステータスPublished - 2002 5 24
外部発表Yes

ASJC Scopus subject areas

  • Neuroscience(all)

これを引用

Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults. / Tajima, Hirohisa; Niikura, Takako; Hashimoto, Yuichi; Ito, Yuko; Kita, Yoshiko; Terashita, Kenzo; Yamazaki, Kazuto; Koto, Atsuo; Aiso, Sadakazu; Nishimoto, Ikuo.

:: Neuroscience Letters, 巻 324, 番号 3, 24.05.2002, p. 227-231.

研究成果: Article

Tajima, H, Niikura, T, Hashimoto, Y, Ito, Y, Kita, Y, Terashita, K, Yamazaki, K, Koto, A, Aiso, S & Nishimoto, I 2002, 'Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults', Neuroscience Letters, 巻. 324, 番号 3, pp. 227-231. https://doi.org/10.1016/S0304-3940(02)00199-4
Tajima, Hirohisa ; Niikura, Takako ; Hashimoto, Yuichi ; Ito, Yuko ; Kita, Yoshiko ; Terashita, Kenzo ; Yamazaki, Kazuto ; Koto, Atsuo ; Aiso, Sadakazu ; Nishimoto, Ikuo. / Evidence for in vivo production of Humanin peptide, a neuroprotective factor against Alzheimer's disease-related insults. :: Neuroscience Letters. 2002 ; 巻 324, 番号 3. pp. 227-231.
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abstract = "An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99{\%} identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.",
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AU - Niikura, Takako

AU - Hashimoto, Yuichi

AU - Ito, Yuko

AU - Kita, Yoshiko

AU - Terashita, Kenzo

AU - Yamazaki, Kazuto

AU - Koto, Atsuo

AU - Aiso, Sadakazu

AU - Nishimoto, Ikuo

PY - 2002/5/24

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N2 - An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.

AB - An unbiased functional screening with brain cDNA library from an Alzheimer's disease (AD) brain identified a novel 24-residue peptide Humanin (HN), which suppresses AD-related neurotoxicity. As the 1567-base cDNA containing the open reading frame (ORF) of HN is 99% identical to mitochondrial 16S ribosomal RNA as well as registered human mRNA, it was elusive whether HN is produced in vivo. Here, we raised anti-HN antibody and found that long cDNAs containing the ORF of HN (HN-ORF) produced the HN peptide in mammalian cells, dependent on the presence of full-length HN-ORF. Immunoblot analysis detected a 3-kDa protein with HN immunoreactivity in the testis and the colon in 3-week-old mice and in the testis in 12-week-old mice. HN immunoreactivity was also detected in an AD brain, but little in normal brains. This study suggests that HN peptide could be produced in vivo, and would provide a novel insight into the pathophysiology of AD.

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KW - Neuronal death

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