Fluorometric Biosniffer Camera "sniff-Cam" for Direct Imaging of Gaseous Ethanol in Breath and Transdermal Vapor

Takahiro Arakawa, Toshiyuki Sato, Kenta Iitani, Koji Toma, Kohji Mitsubayashi

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Various volatile organic compounds can be found in human transpiration, breath and body odor. In this paper, a novel two-dimensional fluorometric imaging system, known as a "sniffer-cam" for ethanol vapor released from human breath and palm skin was constructed and validated. This imaging system measures ethanol vapor concentrations as intensities of fluorescence through an enzymatic reaction induced by alcohol dehydrogenase (ADH). The imaging system consisted of multiple ultraviolet light emitting diode (UV-LED) excitation sheet, an ADH enzyme immobilized mesh substrate and a high-sensitive CCD camera. This imaging system uses ADH for recognition of ethanol vapor. It measures ethanol vapor by measuring fluorescence of nicotinamide adenine dinucleotide (NADH), which is produced by an enzymatic reaction on the mesh. This NADH fluorometric imaging system achieved the two-dimensional real-time imaging of ethanol vapor distribution (0.5-200 ppm). The system showed rapid and accurate responses and a visible measurement, which could lead to an analysis of metabolism function at real time in the near future.

本文言語English
ページ(範囲)4495-4501
ページ数7
ジャーナルAnalytical Chemistry
89
8
DOI
出版ステータスPublished - 2017 4月 18
外部発表はい

ASJC Scopus subject areas

  • 分析化学

フィンガープリント

「Fluorometric Biosniffer Camera "sniff-Cam" for Direct Imaging of Gaseous Ethanol in Breath and Transdermal Vapor」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル