抄録
We cloned the structural gene for monoamine oxidase (maoA) from Klebsiella aerogenes into a pKI212 vector in an maoA mutant strain of K. aerogenes. Deletion analysis and complementation tests of the recombinant plasmid showed that the maoA gene was located entirely within a 4.1-kb segment. In an maoA mutant strain harbouring the cloned maoA gene, synthesis of monoamine oxidase was induced by addition of tyramine and related compounds. Transfer of a plasmid containing the maoA gene into a monoamine oxidase-producing strain of K. aerogenes W70 resulted in about a 30- to 40-fold increase in total production of the enzyme. When cells of K. aerogenes carrying the plasmid containing the maoA gene were grown with tyramine, more than 85% of the monoamine oxidase was produced in soluble form, whereas the parent strain W70 produced most monoamine oxidase as the membrane-bound form.
本文言語 | English |
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ページ(範囲) | 606-610 |
ページ数 | 5 |
ジャーナル | Applied Microbiology and Biotechnology |
巻 | 35 |
号 | 5 |
DOI | |
出版ステータス | Published - 1991 8月 1 |
外部発表 | はい |
ASJC Scopus subject areas
- バイオテクノロジー
- 応用微生物学とバイオテクノロジー