Gene cloning of the maoA gene and overproduction of a soluble monoamine oxidase from Klebsiella aerogenes

Hiroyuki Sugino, Kaname Ishibashi, Masashi Sakaue, Mitsuo Yamashita, Yoshikatsu Murooka

研究成果: Article査読

10 被引用数 (Scopus)

抄録

We cloned the structural gene for monoamine oxidase (maoA) from Klebsiella aerogenes into a pKI212 vector in an maoA mutant strain of K. aerogenes. Deletion analysis and complementation tests of the recombinant plasmid showed that the maoA gene was located entirely within a 4.1-kb segment. In an maoA mutant strain harbouring the cloned maoA gene, synthesis of monoamine oxidase was induced by addition of tyramine and related compounds. Transfer of a plasmid containing the maoA gene into a monoamine oxidase-producing strain of K. aerogenes W70 resulted in about a 30- to 40-fold increase in total production of the enzyme. When cells of K. aerogenes carrying the plasmid containing the maoA gene were grown with tyramine, more than 85% of the monoamine oxidase was produced in soluble form, whereas the parent strain W70 produced most monoamine oxidase as the membrane-bound form.

本文言語English
ページ(範囲)606-610
ページ数5
ジャーナルApplied Microbiology and Biotechnology
35
5
DOI
出版ステータスPublished - 1991 8月 1
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

フィンガープリント

「Gene cloning of the maoA gene and overproduction of a soluble monoamine oxidase from Klebsiella aerogenes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル