Global analysis of shear stress-responsive genes in vascular endothelial cells.

Norihiko Ohura, Kimiko Yamamoto, Sigeru Ichioka, Takaaki Sokabe, Hideki Nakatsuka, Atsushi Baba, Masahiro Shibata, Takashi Nakatsuka, Kiyonori Harii, Youichiro Wada, Takahide Kohro, Tatsuhiko Kodama, Joji Ando

研究成果: Article

108 引用 (Scopus)

抄録

DNA microarray gene expression analysis was conducted in human umbilical vein endothelial cells (HUVECs) and coronary artery endothelial cells (HCAECs) exposed to laminar or turbulent shear stress. Approximately 3% of the total 5600 gene in HUVECs and HCAECs increased their expression more than two-fold or decreased it to less than half the static control in response to an arterial level of laminar shear stress (15 dynes/cm(2) for 24 hours). The proportions of shear-stress-responsive genes decreased to around 2% under the venous level of laminar shear stress (1.5 dynes/cm(2)) in both cell lines. Turbulent shear stress of 1.5 dynes/cm(2) altered the expression of 1.1% of all genes in the HCAECs. Laminar shear stress, but not turbulent shear stress, decreased the expression of a number of genes involved in DNA synthesis and the cell cycle in both HUVECs and HCAECs. Clustering analysis showed a variety of temporal profiles of gene expression in HUVECs exposed to laminar shear stress of 15 dynes/cm(2) for 3, 6, 12, 24, and 48 hours. Turbulent shear stress affected expression of many genes that play a role in vascular remodeling, including genes encoding plasminogen activators and their inhibitor, endothelin-1, transforming growth factor-beta, collagen type IV, and ephrin A1.

元の言語English
ページ(範囲)304-313
ページ数10
ジャーナルJournal of Atherosclerosis and Thrombosis
10
発行部数5
出版物ステータスPublished - 2003
外部発表Yes

Fingerprint

Endothelial cells
Shear stress
Genes
Gene expression
Ephrin-A1
Cells
Plasminogen Inactivators
Gene encoding
Collagen Type IV
DNA
Endothelin-1
Microarrays
Transforming Growth Factor beta

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Internal Medicine
  • Biochemistry, medical

これを引用

Ohura, N., Yamamoto, K., Ichioka, S., Sokabe, T., Nakatsuka, H., Baba, A., ... Ando, J. (2003). Global analysis of shear stress-responsive genes in vascular endothelial cells. Journal of Atherosclerosis and Thrombosis, 10(5), 304-313.

Global analysis of shear stress-responsive genes in vascular endothelial cells. / Ohura, Norihiko; Yamamoto, Kimiko; Ichioka, Sigeru; Sokabe, Takaaki; Nakatsuka, Hideki; Baba, Atsushi; Shibata, Masahiro; Nakatsuka, Takashi; Harii, Kiyonori; Wada, Youichiro; Kohro, Takahide; Kodama, Tatsuhiko; Ando, Joji.

:: Journal of Atherosclerosis and Thrombosis, 巻 10, 番号 5, 2003, p. 304-313.

研究成果: Article

Ohura, N, Yamamoto, K, Ichioka, S, Sokabe, T, Nakatsuka, H, Baba, A, Shibata, M, Nakatsuka, T, Harii, K, Wada, Y, Kohro, T, Kodama, T & Ando, J 2003, 'Global analysis of shear stress-responsive genes in vascular endothelial cells.', Journal of Atherosclerosis and Thrombosis, 巻. 10, 番号 5, pp. 304-313.
Ohura N, Yamamoto K, Ichioka S, Sokabe T, Nakatsuka H, Baba A その他. Global analysis of shear stress-responsive genes in vascular endothelial cells. Journal of Atherosclerosis and Thrombosis. 2003;10(5):304-313.
Ohura, Norihiko ; Yamamoto, Kimiko ; Ichioka, Sigeru ; Sokabe, Takaaki ; Nakatsuka, Hideki ; Baba, Atsushi ; Shibata, Masahiro ; Nakatsuka, Takashi ; Harii, Kiyonori ; Wada, Youichiro ; Kohro, Takahide ; Kodama, Tatsuhiko ; Ando, Joji. / Global analysis of shear stress-responsive genes in vascular endothelial cells. :: Journal of Atherosclerosis and Thrombosis. 2003 ; 巻 10, 番号 5. pp. 304-313.
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AU - Ichioka, Sigeru

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AU - Nakatsuka, Hideki

AU - Baba, Atsushi

AU - Shibata, Masahiro

AU - Nakatsuka, Takashi

AU - Harii, Kiyonori

AU - Wada, Youichiro

AU - Kohro, Takahide

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