Handheld recirculation system and customized media for microfluidic cell culture

Nobuyuki Futai, Wei Gu, Jonathan W. Song, Shuichi Takayama

研究成果: Article

83 引用 (Scopus)

抜粋

A palm-sized microfluidic recirculation system and customized media enable simplified long-term culture and imaging of cells. The combination of bare Braille display modules, a leveled monolithic surface for complete chip mounting, and a transparent heater improved portability, mechanical stability and optical accessibility. Modification of basal culture media with Leibovitz's L-15 medium enabled an incubator-free culture of carbonate-dependent cells by eliminating the need for exogenous carbon dioxide. This capability is demonstrated through time-lapse recording of proliferation of C2C12 myoblasts and MC3T3-E1 osteoblasts for over 2 weeks in ambient atmosphere without medium exchange. The method opens up new possibilities for portable cell culture and for long-term continuous visual monitoring of cells.

元の言語English
ページ(範囲)149-154
ページ数6
ジャーナルLab on a Chip
6
発行部数1
DOI
出版物ステータスPublished - 2006 1 1

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ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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